Centella asiatica (CA) has been widely used as herbal plants. It is a valuable resources. The aim of the present study was to evaluate physiological activities of solvent extracts from CA cultivated in Chungju, Korea (Good tiger care). After preparing water (cold-water, CA-WE; hot-water, CA-HWE) and EtOH extracts (50% EtOH, CA-50E; 70% EtOH, CA-70E), their total polyphenol, flavonoid, and triterpenoid contents, and anti-oxidant activities, and anti-inflammatory activities were examined and compared. CA-70E showed the most potent anti-oxidant activity based on ABTS radical scavenging activity and reducing ability assays, while CA-50E and CA-70E showed the highest DPPH radical scavenging activity. Among major triterpenoid glycosides present in CA, madecassoside and asiaticoside contents were found to be the highest in CA-70E, and madecassic acid and asiatic acid were the highest in CA-50E. In LPS-stimulated RAW 264.7 cells, CA-70E showed the highest TNF-α inhibitory activity, although CA-50E and CA-70E similarly inhibited nitric oxide production. Ethanol extracts significantly inhibited IL-6 and IL-8 production more than water extracts using TNF-α/IFN-γ-stimulated HaCaT cells, indicating its better inhibitory against skin inflammation. Therefore, Chungju-CA EtOH extract, especially 70% EtOH extract, has high physiologically active ingredients and potent anti-oxidant and anti-inflammatory activities, suggesting its industrial application as a functional material.

Abstract
서 론
재료 및 방법
    1. 실험재료 및 추출방법
    2. 폴리페놀 및 플라보노이드 함량 평가
    3. 자유라디칼 소거활성 및 환원력 평가
    4. 세포주 및 배양조건
    5. 항염증 활성 평가
    6. HPLC-DAD를 이용한 triterpenoid의 정량 분석
    7. 통계처리
결과 및 고찰
    1. 병풀추출물의수율과총폴리페놀및플라보노이드함량
    2. 병풀추출물 4종의 항산화 활성
    3. 병풀추출물의 pentacyclic triterpenoid 화합물 함량 비교
    4. 병풀추출물 4종의 항염증 활성
요약 및 결론
References

• 신현영(고려대학교 의생명융합과학과) | Hyun-Young Shin (Dept. of Integrated Biomedical and Life Science, Korea University)
• 김훈(경희대학교 피부생명공학센터) | Hoon Kim (Skin Biotechnology Center, Kyung Hee University)
• 정은진(한국교통대학교 식품영양학전공) | Eun-Jin Jeong (Major in Food and Nutrition, Korea National University of Transportation)
• 김정은(한국교통대학교 식품영양학전공) | Jeung-Eun Kim (Major in Food and Nutrition, Korea National University of Transportation)
• 이경행(한국교통대학교 식품영양학전공) | Kyeong-Haeng Lee (Major in Food and Nutrition, Korea National University of Transportation)
• 배윤정(한국교통대학교 식품영양학전공) | Yun-Jeong Bae (Major in Food and Nutrition, Korea National University of Transportation)
• 유광원(한국교통대학교 식품영양학전공) | Kwang-Won Yu (Major in Food and Nutrition, Korea National University of Transportation) Corresponding author