2019년 1월부터 11월까지 서울약령시장에서 유통되는 식·약 공용 농산물 총 187건을 대상으로 곰팡이독소 동시 다성분 SPE 컬럼으로 정제 후 LC-MS/MS로 분석하여 곰팡이독소 8종의 동시분석법 유효성을 검증하고, 확립된 분석법으로 곰팡이독소 오염도 파악 및 위해평가를 실시하였다. LC-MS/MS를 이용한 동시분석법의 유효성 검증은 매질효과, 직선성, 검출한계, 정량한계, 정확성 및 정밀성으로 하였다. 매질 보정 검량선의 상관계수(r2)는 0.9999이상의 우수한 직선성을 보였고, 검출한계는 0.02-0.11 μg/ kg였고, 정량한계는 0.06-0.26 μg/kg였고, 회수율은 81.2- 118.7%였고, 상대표준편차는 0.33-8.90%로 우수한 재현성을 나타냈다. 확립된 분석법으로 검사한 결과 기준이 설정된 아플라톡신은 B1이 1.18-7.29 μg/kg (기준: 총 아플라톡신 15.0 μg/kg이하, B1 10.0 μg/kg이하)으로 기준 이내로 검출되었고, 아플라톡신 B2, G1 및 G2는 검출되지 않았다. 기준이 미설정된 곰팡이독소는 푸모니신(0.84-14.25 μg/ kg) 오크라톡신 A (0.76-17.42 μg/kg) 및 제랄레논(1.73- 15.96 μg/kg)이 검출되었다. 위해평가 결과 아플라톡신 B1의 1일 인체노출량은 0.00052 μg/kg b.w./day였고, 푸모니신 및 제랄레논의 일일섭취한계량 대비 각각 0.04%, 0.24% 였고, 오크라톡신 A의 주간섭취한계량 대비 4.76%로 우리나라 국민들이 식·약 공용 농산물 섭취로 인한 곰팡이 독소 위해도는 안전한 것으로 평가되었다.

For this study, we surveyed concentrations of 8 mycotoxins (aflatoxin B1, B2, G1, G2, ochratoxin A, fumonisin B1, B2 and zearalenone) in agricultural products used for food and medicine by liquid chromatographytandem mass spectrometry and conducted a risk assessment. Samples were collected at the Yangnyeong Market in Seoul, Korea, between January and November 2019. Mycotoxins were extracted from these samples by adding 0.1% formic acid in 50% acetonitrile and cleaned up by using an ISOLUTE Myco cartridge. The method was validated by assessing its matrix effects, linearity, limit of detection (LOD), limit of quantification (LOQ), recovery and precision using four representative matrices. Matrix-matched standard calibration was used for quantification and the calibration curves of all analytes showed good linearity (r2>0.9999). LODs and LOQs were in the range of 0.02-0.11 μg/kg and 0.06-0.26 μg/kg, respectively. Sample recoveries were from 81.2 to 118.7% and relative standard deviations lower than 8.90%. The method developed in this study was applied to analyze a total of 187 samples, and aflatoxin B1 was detected at the range of 1.18-7.29 μg/kg (below the maximum allowable limit set by the Ministry of Food and Drug Safety, MFDS), whereas aflatoxin B2, G1 and G2 were not detected. Mycotoxins that are not regulated presently in Korea were also detected: fumonisin (0.84-14.25 μg/kg), ochratoxin A (0.76-17.42 μg/kg), and zearalenone (1.73- 15.96 μg/kg). Risk assessment was evaluated by using estimated daily intake (EDI) and specific guideline values. These results indicate that the overall exposure level of Koreans to mycotoxins due to the intake of agricultural products used for food and medicine is unlikely to be a major risk factor for their health.

ABSTRACT
Materials and Methods
    재료
    시약 및 기구
    실험방법
    분석법 유효성 검증
    위해평가
Results and Discussio
    LC-MS/MS 조건 확립
    시험법 유효성검증
    식·약 공용 농산물의 곰팡이독소 오염현황
    식·약 공용 농산물의 위해평가
국문요약
References

• 최수정(서울시보건환경연구원) | Su-Jeong Choi (Seoul Metropolitan Government Research Institute of Public Health and Environment) Correspondence to
• 고숙경(서울시보건환경연구원) | Suk-Kyung Ko (Seoul Metropolitan Government Research Institute of Public Health and Environment)
• 박영애(서울시보건환경연구원) | Young-Ae Park (Seoul Metropolitan Government Research Institute of Public Health and Environment)
• 정삼주(서울시보건환경연구원) | Sam-Ju Jung (Seoul Metropolitan Government Research Institute of Public Health and Environment)
• 최은정(서울시보건환경연구원) | Eun-Jung Choi (Seoul Metropolitan Government Research Institute of Public Health and Environment)
• 김은정(서울시보건환경연구원) | Eun-Jung Kim (Seoul Metropolitan Government Research Institute of Public Health and Environment)
• 황인숙(서울시보건환경연구원) | In-Sook Hwang (Seoul Metropolitan Government Research Institute of Public Health and Environment)
• 신기영(서울시보건환경연구원) | Gi-Young Shin (Seoul Metropolitan Government Research Institute of Public Health and Environment)
• 유인실(서울시보건환경연구원) | In-Sil Yu (Seoul Metropolitan Government Research Institute of Public Health and Environment)
• 신용승(서울시보건환경연구원) | Yong-Seung Shin (Seoul Metropolitan Government Research Institute of Public Health and Environment)