현재 양송이 종균은 1980년대 개발된 방법으로 제조되고 있어, 양송이 종균 품질 향상을 위한 새로운 제조 방법을 개발하였다. 그 결과 균사체량이 5.92±0.52 g/L로 가장 많이 배양된 CDB(compost dextrose broth) 배지에서 24oC에서 120 rpm으로 진탕 배양(24시간/일) 하는 통기 식(2.5 L/min) 액체배양법으로 배양기간 10일이 액체 접종원 사용으로 양호했으며, 양송이 밀 배지 종균 생산은 밀 배지 50 g당 액체 접종원 1 ml을 적정 접종량으로 하여 10일 배양하면 종균의 뭉침과 흔드는 작업없이 적절하게 배양되었음을 보고한다.

Currently, the spawn of the mushroom Agarcus bisporus is produced by a method developed in the 1980s, and anew spawning method needs to be developed to improve the quality of the spawn. In this study, the condition for a maximum mycelium weight(5.92±0.52 g/L) was shaking culture (24 hours/day) at 24°C and 120 rpm in CDB (compost dextrose broth). Based on this, the ventilated liquid culture method (2.5 L/min) was cultured for 10 days. This method was appropriate, andwhen the inoculum was cultured at 50 g/mL for about 10 days, it was cultured well without agglomeration and shaking of seed.

• 오연이(농촌진흥청 국립원예특작과학원 버섯과) | Youn-Lee Oh (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) Corresponding author
• 장갑열(농촌진흥청 국립원예특작과학원 버섯과) | Kab-Yeul Jang (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
• 오민지(농촌진흥청 국립원예특작과학원 버섯과) | Min Ji Oh (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
• 임지훈(농촌진흥청 국립원예특작과학원 버섯과) | Ji-Hoon Im (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)