In order to understand the vegetative role of Glycine soja, we studied the basic physiological characteristics between Glycine soja and Glycine max. For this study, the light intensity (μmol m-2 s-1) on leaf surface, leaf temperature (℃), transpiration rate (mmol m-2 s-1), photosynthetic rate (μmol m-2 s-1), substomatal CO2 partial pressure (vpm) of Glycine soja and Glycine max were measured, and the quantum yield, photosynthesis rate per substomatal CO2 partial pressure were calculated. In the results of simple regression analysis, the increasing quantum yield decreases leaf temperature both of Glycine soja and Glycine max and the increasing leaf temperature decreases transpiration rate in case of Glycine soja. However, in case of Glycine max, the increasing leaf temperature decreases substomatal CO2 partial pressure, photosynthetic rate, and photosynthetic rate per substomatal CO2 partial pressure as well as transpiration rate. Also, increasing transpiration rate increases substomatal CO2 partial pressure while decreases photosynthetic rate per substomatal CO2 partial pressure. Thus, Glycine soja is relatively more easily adaptable to severe environments with low soil nutrients and high light levels. Compared to Glycine max susceptible to water loss due to a water-poor terrestrial habitat, the physiological traits of Glycine soja has a high average transpiration rate and are less susceptible to water loss will act as a factor that limits the habitat according to soil moisture.