We used seawater temperature data, measured in the Garolim Bay, to analyze temperature variation on an hourly and daily basis. Lagrange’s interpolation using before and after data was applied to restore nonconsecutive missing temperature data. The estimated error of the data restoration was 0.11oC. Spectral analyses of seawater temperature showed significant periodicities of approximately 12.4 h (semidiurnal tide) and 15.0 d (long-period tide), which is close to those of M2 and Mf partial tides. Variation in seawater temperature was correlated more with tidal height than with air temperature around the Garolim Bay. In June and December, when the seawater temperature difference between the inside and outside of the Garolim Bay was very large, the periodicities of 12.4 h and 15.0 d were highly prominent. These results indicate that the exchange of seawater between the inside and outside of the Garolim Bay induced variations in seawater temperature owing to tide. Understanding temperature variation because of tide helps to prevent abnormal mortality of cultured fish and to predict seawater temperature in the Garolim Bay.