Oligosaccharide-producing lactic acid bacteria were isolated from young radish and identified as Weissella cibaria YRK005. Oligosaccharides from W. cibaria YRK005 were purified using size-exclusion high-performance liquid chromatography, and their structure was determined. The oligosaccharides consisted of glucose only, 1 H-nuclear magnetic resonance analysis revealed that the oligosaccharides consisted of 93.8% α-1,6 glycosidic linkages and 6.2% α-1,4 glycosidic linkages, and molecular weight was determined to be 1.1210 2 Da. When the oligosaccharides' prebiotic effect on probiotic strains was examined, the oligosaccharides promoted the growth of four probiotic strains, Bifidobacterium adolescentis, Lactobacillus acidophilus, Lactococcus lactis, and Lactobacillus pentosus, compared to fructo-oligosaccharides as a reference. These results indicated that oligosaccharides from W. cibaria YRK005 could be used as potential prebiotics.

Abstract
Introduction
Materials and Methods
    Bacterial isolation and cultivation
    Identification of oligosaccharide-producing LAB
    Production and purification of oligosaccharidesfrom W. cibaria YRK005
    Determination of the oligosaccharide structure
    Probiotic strains and culture media
    Media for probiotic strains for the determination ofthe prebiotic effect
    Growth of probiotic strains for the determination ofthe prebiotic effect
    Statistical analysis
Results and Discussion
    Screening of oligosaccharide-producing LAB
    Identification of oligosaccharide-producing strain
    Determination of the structure of oligosaccharidesfrom W. cibaria YRK005
    Analysis of molecular mass and monosaccharidecomposition of oligosaccharides
    Determination of glycosidic linkages in oligosaccharidesby enzymatic digestion
    Determination of glycosidic linkages in oligosaccharidesby 1H-NMR spectroscopy
    Prebiotic effects of oligosaccharides
References

• Jisun Park(Department of Food Science and Biotechnology, Gachon University)
• Young-Seo Park(Department of Food Science and Biotechnology, Gachon University) Corresponding author