본 연구는 추출용매 및 주정에 의한 탈지 및 비탈지 포도종자 추출물의 수율, 항산화활성 및 총페놀함량을 분석하였으며, 각종 pH에서 처리된 추출물의 저장시 항산화력 변화를 살펴보았다. 추출수율은 탈지 및 비탈지 처리한 포도종자 분말 모두 에탄올로 에서 6시간 추출하였을 때 각각 로 다른 유기용매에 비하여 가장 높은 것으로 나타났으며, methanol, acetone-acetic acid 및 acetone 순으로 나타났으며 ethanol 탈지추
This study was conducted to determine the yield, free radical scavengering effect and total phenol contents of various solvent fractions on the crude and defatted grape seed extract during storage. The optimal condition for the extraction yield, free radical scavengering effect and total phenol contents was ethanol for 6 hour at . The extraction yield for crude and defatted grape seed at optimal condition was , respectively. Also, the strongest free radical scavengering effect with was observed in ethanol of defatted grape seed extracted for 6 hour at . Similar result was observed in total phenol contents of defatted grape seed. The ethyl acetate fraction obtained from ethanol extract of defatted grape seed showed the strongest RC50() compared to other organic fractions. Free radical scavengering effect of crude and defatted grape seed extracts treated with alkali condition(pH 10) was reduced compared to that of acidic condition(pH 2) during storage far 1 month at . Overall, more stronger free radical scavengering effect and higher total phenol contents in defatted grape seed extracts was observed than that of crude grape seed.