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pp.275-281
우슬이 민간요법으로 관절염 치료에 우수한 한약재로 알려진 바 우슬 추출물이 cathepsin B 에 대한 저해력을 검토하기 위하여 각종 용매로 우슬 추출물을 순차 분획하고 분획 추출물에 대하여 cathepsin B에 대한 저해활성을 검토하였으며 우슬의 지표물질인 20-hydroxy ecdysone이 추출분획에 검출되는지 TLC 및 HPLC를 이용하여 분석하였으며 우슬의 지표물질이 cathepsin B에 대한 저해활성여부를 확인한바 다음과 같은 결
This study was carried out to investigate the cathepsin B inhibition effect by Achyranthes japonica N. root extract in vitro. The methanol/(4:1, v/v) extract was fractionated by ethyl acetate(F1), chloroform(F2), chloroform/methanol(3:1, v/v)(F3) and methanol(F4). The yield of F4 in Achyranthes japonica N. root was . As an index material of Achyranthes japonica N. root, 20-hydroxy ecdysone was detected by TLC, and HPLC and it's content was . Three isolates(F1, F3, F4) showed the cathepsin B inhibition activity, and F4 showed the highest inhibition activity among them. In the inhibition activity on cathepsin B, leupeptin, 20-hydroxy ecdysone and F4(at the same concentration of 20-hydroxy ecdysone.) were 92, 88 and on BANA(-benzoyl-DL-arginine -naphthylamide) substrate, and 62, 36 and on CLN(-CBZ(carbobenzlyoxy)-L-lysine p-nitrophenyl ester HCI) substrate, respectively.
