To understand how to efficiently observe the biomass and community of phytoplankton, phytoplankton sampling was carried out from June to October 2019 at the Yeongju dam sediment control reservoir (YJ) and Bohyeonsan dam reservoir (BH1 and BH2). The results derived from microscopic observation, such as the conventional phytoplankton qualitative/quantitative analysis, and from the CHEMTAX method based on the pigments, were compared. The relative contribution of phytoplankton, calculated by the microscopy and CHEMTAX methods, showed a significant difference in all four classes: cryptophyta, chlorophyta, cyanobacteria, and diatoms. In addition, the correlation between the two observation methods was poor. This might be caused by methodological differences in microscopy that do not consider the varying cell sizes among phytoplankton species. In this study, by converting the cells into carbon, the slope between both carbon biomasses based on microscopy and CHEMTAX was improved close to the 1 : 1 line, and the y-intercept was closer to 0 for cryptophyta and diatoms. For cyanobacteria, the slope increased, the y-intercept decreased, and the plot approached 1 : 1 although the correlation coefficients were not improved in all classes. The present study suggests that application of CHEMTAX based on pigment analysis could be a possible approach to efficiently determine the relative carbon proportions of individual classes of phytoplankton community composition.

서 론
재료 및 방 법
    1. 식물플랑크톤 채집 및 전처리
    2. 식물플랑크톤 현미경 관찰
    3. 식물플랑크톤 지표색소 분석
    4. CHEMTAX program
    5. 탄소함량 기반 생체량 산출 (biomass, μgC L-1)
    6. 통계분석
결 과
    1. 식물플랑크톤 군집 구조
    2. 식물플랑크톤 지표색소 및 군집 기여율 분석 (HPLCCHEMTAX
    3. 식물플랑크톤 현미경관찰법과 HPLC-CHEMTAX법비교
고 찰
적 요

• 김도균(한양대학교 해양융합공학과) | Dokyun Kim (Department of Marine Sciences and Convergent Technology, Hanyang University, Ansan 15588, Republic of Korea)
• 최지수(한양대학교 해양융합공학과) | Jisoo Choi (Department of Marine Sciences and Convergent Technology, Hanyang University, Ansan 15588, Republic of Korea)
• 오혜지(경희대학교 환경학및환경공학과) | Hye-Ji Oh (1Department of Environmental Science and Engineering, Kyung Hee University, Yongin 17104, Republic of Korea)
• 장광현(경희대학교 환경학및환경공학과) | Kwang-Hyeon Chang (Department of Environmental Science and Engineering, Kyung Hee University, Yongin 17104, Republic of Korea)
• 최광순(한국수자원공사 K-water연구원) | Kwangsoon Choi (K-water Research Institute, Daejeon 34045, Republic of Korea)
• 신경훈(한양대학교 해양융합공학과) | Kyung-Hoon Shin ((Department of Marine Sciences and Convergent Technology, Hanyang University, Ansan 15588, Republic of Korea) Corresponding author