영남 평야지에서는 추석전 햅쌀 출하를 목적으로 조기재배나 극조기재배가 증가하고 있는 추세이다. 그러나 극조기 벼 수확 이후에 후작물로 적합한 작목이 없어서 휴경을 하는 농가가 많아 적합한 작부체계 개발을 위한 재배법 개발이 필요한 실정이다. 따라서 본 시험은 극조기 벼 수확 후 이모작 작부체계에 적합한 벼 품종 및 재배시기 설정을 위해 수행하였다. 조생종 14개 품종을 4월 7일, 4월 14일, 4월 21일, 4월 28일에 각각 이앙하였을 때, 극조기 벼 이모작을 위한 기준 출수기인 7월 10일 이전에 출수가능한 품종은 백일미, 주남조생, 중모1032, 진옥이었다. 이 품종들의 적산온도로 추정한 적정 이앙시기는 4월 10일에서 4월 20일 사이로, 이 시기에 이앙할 경우 8월 20일 이내에 수확이 가능하여 8월 하순 후작물과의 작기연결성에 유리한 적합 품종으로 판단된다.

본 연구는 발효노니 다당체 추출물(Vitalbos)을 건강기능 식품 소재로 활용하기 위해 DAA, 총당 함량, 단당류 3종 (galacturonic acid, glucose 및 galactose)을 지표성분으로 설정하고, 지표성분에 대한 효과적인 분석법 설정 및 검 증을 위해 수행되었다. 기존에 보고된 분석법 검증 방법 을 수정하여 특이성, 직선성, 정밀성, 정확성, 검출한계 (LOD) 및 정량한계(LOQ)를 고성능 액체크로마토그래피와 페놀-황산법을 이용하여 측정하였다. 그 결과 DAA 및 단 당류 3종의 표준용액과 Vitalbos의 머무름 시간이 일치하 였으며 스펙트럼 또한 동일하여 분석법의 특이성을 확인 하였다. 지표성분의 검량선 상관계수(R2)는 0.9995-0.9998 범위로 0.99 이상의 우수한 직선성을 나타냈다. Intra-day 및 inter-day 정밀도는 0.14-3.01%의 범위로 5% 미만의 우 수한 정밀도를 나타냈고 회수율은 95.13-105.59% 범위에 서 우수한 정확도를 보였다. DAA 분석의 LOD와 LOQ는 각각 0.39 μg/mL 및 1.18 μg/mL이었으며 총당 함량의 LOD 및 LOQ는 각각 0.84 μg/mL 및 2.55 μg/mL로 측정 되었다. 단당류 3종에 대한 LOD는 0.48-0.81 μg/mL의 범 위였으며, LOQ는 1.45-2.44 μg/mL 범위에서 정량분석이 가능한 것으로 나타났다. 분석법 검증 결과, 특이성, 직선 성, 정밀성 및 정확성 모두 우수한 분석법임을 검증하였 으며, LOD와 LOQ 또한 Vitalbos 분석에 적합하였음을 확 인하였다. 검증된 분석법을 이용하여 Vitalbos의 지표 성분 함량을 측정하였을 때, DAA, 총당 함량, galacturonic acid, glucose 및 galactose의 함량은 각각 2.31±0.06 mg/dry weight g, 475.92±5.95 mg/dry weight g, 72.83±1.05 mg/dry weight g, 71.63±2.44 mg/dry weight g 및 67.30±2.31 mg/dry weight g으로 측정되었다. 본 연구에서 검증된 분석법을 사용했을 때 Vitalbos의 지표성분 3종에 대하여 우수한 재현성으로 정 량분석이 가능하였으며, 건강기능식품 소재로의 품질관리에 기여할 수 있을 것으로 판단된다.

A sediment control dam is an artificial structure built to prolong sedimentation in the main dam by reducing the inflow of suspended solids. These dams can affect changes in dissolved organic matter (DOM) in the water body by changing the river flow regime. The main DOM component for Yeongju Dam sediment control of the Naeseongcheon River was analyzed through 3D excitation-emission matrix (EEM) and parallel factor (PARAFAC) analyses. As a result, four humic-like components (C1~C3, C5), and three proteins, tryptophan-like components (C2, C6~C7) were detected. Among DOM components, humic-like components (autochthonous: C1, allochthonous: C2~C3) were found to be dominant during the sampling period. The total amount of DOM components and the composition ratio of each component did not show a difference for each depth according to the amount of available light (100%, 12%, and 1%). Throughout the study period, the allochthonous organic matter was continuously decomposing and converting into autochthonous organic matter; the DOM indices (fluorescence index, humification index, and freshness index) indicated the dominance of autochthonous organic matter in the river. Considering the relative abundance of cyanobacteria and that the number of bacteria cells and rotifers increased as autochthonous organic matter increased, it was suggested that the algal bloom and consequent activation of the microbial food web was affected by the composition of DOM in the water body. Research on DOM characteristics is important not only for water quality management but also for understanding the cycling of matter through microbial food web activity.

To understand how to efficiently observe the biomass and community of phytoplankton, phytoplankton sampling was carried out from June to October 2019 at the Yeongju dam sediment control reservoir (YJ) and Bohyeonsan dam reservoir (BH1 and BH2). The results derived from microscopic observation, such as the conventional phytoplankton qualitative/quantitative analysis, and from the CHEMTAX method based on the pigments, were compared. The relative contribution of phytoplankton, calculated by the microscopy and CHEMTAX methods, showed a significant difference in all four classes: cryptophyta, chlorophyta, cyanobacteria, and diatoms. In addition, the correlation between the two observation methods was poor. This might be caused by methodological differences in microscopy that do not consider the varying cell sizes among phytoplankton species. In this study, by converting the cells into carbon, the slope between both carbon biomasses based on microscopy and CHEMTAX was improved close to the 1 : 1 line, and the y-intercept was closer to 0 for cryptophyta and diatoms. For cyanobacteria, the slope increased, the y-intercept decreased, and the plot approached 1 : 1 although the correlation coefficients were not improved in all classes. The present study suggests that application of CHEMTAX based on pigment analysis could be a possible approach to efficiently determine the relative carbon proportions of individual classes of phytoplankton community composition.

지구 온난화에 따른 기온 상승, 보나 댐의 건설로 인한 체류시간의 변화는 식물플랑크톤의 이상증식을 일으키며 이로 인한 수계의 부영양화, 녹조 현상은 상수원 수질 악화에 영향을 미쳐 심각한 사회적 문제로 대두되고 있다. 본 연구와 같이 일차생산력과 환경요인들의 상관관계를 확인하는 것은 일차생산력의 조절인자 파악에 매우 효과적이다. 또한 HPLC-CHEMTAX 분석 방법은 간편하며 짧은 시간 안에 식물플랑크톤의 색소 비 값을 기반으로 각 군집의 상대적인 기여도 추정이 가능하다. 이러한 연구 방법들을 적용할 경우 수 생태 건강성 평가 및 식물플랑크톤 이상증식에 영향을 주는 군집조성을 빠르게 파악할 수 있어 조류 예보제 및 수계관리에 있어 효과적으로 활용될 수 있을 것으로 판단된다.

층면구조 안정성 다이어그램은 층면구조가 주어진 입도와 유속에서 나타나는 층면구조의 모양과 크기를 지시한다. 이 다이어그램은 대부분 수조실험에 의해 획득한 실험 데이터를 기반으로 작성되었다. 일반적으로, 수조실험은 입자의 크기와 유속사이의 관계를 이해하기 위해 분급이 좋고 단일입도의 분포를 보이는 퇴적물을 이용하여 수행되었다. 이 다이어그램에 의하면, 세립사와 중립사 퇴적물 표면에서 유속이 빨라지면서, 평행층에서 연흔이 형성되기 시작한다. 이 연구의 목적은 층면구조 안정성 다이어그램의 결과가 실험을 통하여 잘 재현되는지를 확인하고, 분급이 좋은 퇴적물과 달리 분급이 불량한 경우인 이정 입도 분포를 보이는 사질 퇴적물에서도 잘 재현되는지 확인하는 것이다. 본 연구 실험 결과는 2D 연흔이나 3D 연흔 층면구조가 형성되기 위해서, 분급이 불량한 퇴적물의 경우에, 분급이 좋은 퇴적물보다 더 높은 유체의 유속과 전단응력이 필요하다는 것을 보여주고 있다. 탄산염 퇴적물은 수력학적 분급작용이 활발하지 않으며, 퇴적물의 구성이 알로켐과 기질로 이루어지는 이정 입도 분포를 보이는 퇴적물로서 일반적으로 분급이 불량한 특징을 가지고 있다. 따라서, 실험의 결과는 탄산염 퇴적물에서 층면구조 형성을 위해 쇄설성 퇴적환경의 퇴적물 보다 더 높은 유속이 필요할 수 있음을 제안하고 있다. 분급이 불량한 퇴적물 입자가 침식되어 이동기 위해 더 높은 에너지와 유속이 필요하다는 것은 분급 효과, 마찰 효과, 안정성 효과, 갑옷 효과 등이 복합적으로 작용한 결과로 설명될 수 있을 것이다. 본 연구는 예비적 고찰로서, 이어지는 연구를 통해 이러한 현상을 과학적으로 설명하고 입도와 층면구조 형성의 상관성을 보다 정교하게 규명하고자 한다.

To identify rice (Oryza sativa L.) cultivars suitable for Italian ryegrass (Lolium multiflorum Lam.)–rice double cropping systems, we investigated the yield and grain quality of four different midseason maturing rice cultivars (‘Daebo’, ‘Haepum’, ‘Haiami’, and ‘Samdeog’) and four midseason-to-late maturing rice cultivars (‘Hyunpoom’, ‘Saeilmi’, ‘Saenuri’, and ‘Samkwang’) in single rice cropping and Italian ryegrass–rice double cropping systems in paddy fields of Miryang, South Korea. We found that organic matter and available P2O5 content slightly decreased, whereas Na content increased, in the soil where Italian ryegrass was cultivated during winter compared to that in the soil that remained fallow during winter. The pH, electrical conductivity, organic matter, and contents of K+, Ca2+, Mg2+, and Na+ decreased, whereas the available P2O5 content slightly increased, in the soil where rice was harvested in both single and double cropping systems. However, compared to the optimum soil conditions for rice cultivation, available P2O5 and K+ content were high and Mg2+ content was low in both single and double cropping systems. At the heading stage, the culm length and leaf color slightly increased in most of the rice cultivars, whereas the panicle length and number slightly decreased, in the double cropped system. After harvesting, spikelet number and milled rice yield did not show a significant difference between single and double cropping systems. However, the ripened grain rate and weight per thousand grains increased slightly in the ‘Saeilmi’ and ‘Samkwang’ cultivars but remained either stable or slightly low in other cultivars in the double cropping system. The milled rice yield was high (> 500 kg/10a) in ‘Daebo’ and ‘Haepum’ among midseason maturing rice cultivars, and in ‘Saeilmi’ and ‘Saenuri’ among midseason-to-late maturing rice cultivars, in both single and double cropping systems. The head rice rate was high in midseason maturing rice cultivars in the double cropping systems, reaching > 70% in ‘Haepum’ and ‘Haiami’ cultivars, whereas it decreased in most midseason-to-late maturing rice cultivars (excluding ‘Samkwang’ cultivar), in double cropping systems. Particularly, it exceeded > 70% in the ‘Saenuri’ cultivar in both single and double cropping systems. The protein content in milled rice increased, whereas the amylose content either remained stable or slightly increased, in double cropping systems. The Toyo taste value decreased in all midseason-to-late maturing rice cultivars and slightly increased in the ‘Daebo’ and ‘Haiami’ cultivars among midseason maturing rice cultivars in double cropping systems. However, Toyo taste values in the ‘Haepum’, ‘Haiami’, and ‘Saenuri’ cultivars exceeding > 80% in both single and double cropping systems. Therefore, we recommend ‘Haepum’, ‘Haiami’, and ‘Saenuri’ cultivars as candidates for Italian ryegrass–rice double cropping systems due to high yield, head rice rate, and Toyo taste value.

The physicochemical properties of soil and the yield and quality of rice (Oryza sativa L. cv. Sailmi) were assessed using Italian ryegrass (Lolium multiflorum Lam. cv. Kowinearly)-rice double cropping systems in the paddy fields at Goseong and Miryang in southern Korea. The average temperatures during the ripening period were approximately 1 °C higher than the optimal temperature for rice ripening and the sunshine duration was reduced by frequent rainfall. Consequently, it was slightly below the optimal conditions required for rice ripening. In the soil at Goseong, winter Italian ryegrass cropping increased the pH, electrical conductivity, and the contents of organic matter, total nitrogen (T-N), available P2O5, K, Ca, and Mg than winter fallowing. Particularly, the contents of T-N and available P2O5increased significantly. In the soil at Miryang, Italian ryegrass slightly increased the electrical conductivity and the T-N, Mg, and Na contents. Therefore, winter Italian ryegrass cropping improved the physicochemical properties of paddy soils; however, Italian ryegrass-rice double cropping slightly reduced the culm length at both Goseong and Miryang, without markedly changing the panicle length or number compared to fallow-rice cropping. Furthermore, at Goseong, Italian ryegrass-rice double cropping slightly decreased the spikelet number and milled rice yield, and increased the ripened grain rate; however, at Miryang, contrasting results were observed. In addition, fallow-rice cropping revealed no differences in the head rice or opaque rice rates. The protein content was slightly increased in Italian ryegrass-rice double cropping, without significant changes in the amylose content or Toyo value, compared to that in fallow-rice cropping. The peak and breakdown viscosities were slightly decreased. These results indicate that winter Italian ryegrass cropping might alter rice taste but may not exhibit remarkable negative effects on rice cultivation. Therefore, Italian ryegrass-rice double cropping system is recommended for paddy fields in southern Korea. Nevertheless, to increase the rice yield and quality, fertilization standards for rice cropping that consider the changes in the T-N and organic matter contents in paddy fields caused by winter Italian ryegrass cropping need to be established.

To develop an environment-friendly fungicide for controlling tomato wilt diseases, antifungal active substance was isolated Rheum australe D. Don roots against Fusarium oxysporum f. sp. lycopersici, a pathogen of tomato wilt, in this study. Methanol extract obtained from Rheum australe roots was successively fractionated with hexane, chloroform, ethyl acetate, butanol and water. The ethyl acetate fraction, which showed the highest antifungal activity, was separated by column chromatography, and 60 subfractions were obtained. The 60 subfractions were anlayzed for antifungal activities by bioassay. The active compound was identified as 5-[(E)-2- (3-hydroxy-4-methoxyphenyl)ethenyl]benzene-1,3-diol (rhapontigenin) by NMR and GC-MS analysis. As a result of testing antifungal activity of rhapontigenin against Fusarium oxysporum, EC50 of rhapontigenin was showed strong antifungal activity at 7.48 mg/L. Therefore, this study showed that the Rheum australe roots extract can be a potential candidate which is a environment-friendly fungicide against Fusarium oxysporum.