Raphanus sativus var. hortensis f. raphanistroides Makino (Korean wild radish [WR]) are root vegetables belonging to the Brassicaceae family. These radish species mostly grow in sea areas in Asia, where they have been traditionally used as a medicinal food to treat various diseases. To investigate the effect of WR on neuronal cell death in SH-SY5Y cells, betaamyloid was used to develop the cell death model. WR attenuated neuronal cell death in SH-SY5Y and regulated the mitogen-activated protein kinase (MAPK) signaling. WR extract also inhibited acetylcholinesterase inhibitor activity. Additionally, the WR treatment group ameliorated the behavior of the memory-impaired mice in a scopolamine-induced mouse model. In the behavior test, WR treated mice showed shorter escape latency and swimming distance and improved the platform-crossing number and the swimming time within the target quadrant. Furthermore, WR prevented histological loss of neurons in hippocampal CA1 regions induced by scopolamine. This study shows that WR can prevent memory impairment which may be a crucial way for the prevention and treatment of memory dysfunction and neuronal cell death.

Abstract
I. 서 론
II. 연구 내용 및 방법
    1. 연구재료
    2. 갯무 추출물(WR 추출물) 제조
    3. 세포배양 및 neuronal toxicity 모델 확립
    4. 단백질 발현 측정
    5. 아세틸콜린 분해효소 저해 활성 측정
    6. 인지기능 장애 동물 모델 확립
    7. 모리스 수중 미로 장치 및 절차
    8. 해마 세포 밀집도 측정
    9. Statistics
III. 결과 및 고찰
    1. WR 추출물의 뉴런 세포 사멸 억제 효과 검증
    2. WR 추출물의 아세틸콜린 분해효소 저해 효능
    3. WR 추출물의 세포성장 및 분화에 관여하는 MAPK 키나아제 활성 조절 효능
    4. 기억력에 대한 WR 추출물의 효과
    5. 해마의 신경세포 밀도에 대한 WR 추출물의 효과
IV. 요약 및 결론
References

• 허진영(한국식품연구원) | Jinyoung Hur (Korea Food Research Institute) Corresponding author
• 최상윤(한국식품연구원) | Sang Yoon Choi (Korea Food Research Institute)
• 염미정(경희대학교 침구경락학센터) | Mijung Yeom (College of Korean Medicine, Kyung Hee University)