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A Study on the Diversity and Enzyme Productivity of Mesophilic and Thermophilic Bacteria from the Arable Soil Fertilized with Modified Peat Moss KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/414565
pp.84-90
Natural environmental resources are considered a prospective source of microorganisms capable of producing biocatalysts with great potential in industrial areas. Arable soil fertilized with peat moss is a habitat for various microorganisms. The present research focused on the isolation and identification of hydrolase-producing bacteria that thrive at a broad temperature range. In this study, a total of 33 strains were isolated from arable soil fertilized with peat moss (Silla Garden in Busan, South Korea). The isolated bacteria were mesophiles and thermophiles with a wide temperature range. Taxonomic identification showed that the isolated strains belonged to 2 phyla, 5 families, 10 genera, and 24 species. Subsequently, the isolated strains were screened for hydrolase (amylase, lipase, and protease) activity. All isolates possessed activity of at least one enzyme and six bacterial isolates produced combined extracellular enzymes. Diversity of soil bacteria species in the present study suggest the potential of soil bacteria in the various industrial applications.
Introduction
Materials and Methods
Isolation and cultivation of bacteria
Phylogenetic analysis of 16S rRNA sequence
Hydrolytic enzyme activity analysis
Results and Discussion
Isolation and cultivation of bacteria
Phylogenetic analysis of 16S rRNA sequence
Hydrolytic enzyme activity analysis
Acknowledgements
References
