In this study, the quality characteristics of kimchi, such as its salinity, pH, and acidity, were measured and compared, and the HT-29 human colon cancer cells were used to show the anticancer effects of kimchi. The kimchi samples used herein included standard kimchi (SK), turnip kimchi (TK), and turnip-powder-added kimchi (TPK). The measured pH and acidity of TK and TPK showed no significant differences with those of SK. Compared to SK and TK, TPK had higher DPPH scavenging activity and higher total flavonoid content, confirming its antioxidant activity. The cancer cell growth inhibition rates of TK and TPK were significantly higher than that of SK. In HT-29 cells treated with TPK, the mRNA expression of Bcl-xL, an anti-apoptosis-related gene, was lower, and the mRNA expressions of the apoptosis-related genes Bax, Bad, and caspase-9 were higher. TPK showed significantly higher levels of mRNA expressions for the cell-cycle-related genes p53 and p21 than the other samples, in addition to suppression effects on cancer cell proliferation. Compared to SK, TK and TPK suppressed the growth of colon cancer cells and showed higher anticancer effects. Therefore, it is shown that kimchi with added turnip powder had high anticancer effects.

Abstract
서 론
재료 및 방법
    1. 김치시료준비
    2. 순무김치 추출물 제조
    3. 염도 측정
    4. pH와 산도 측정
    5. 순무김치의 DPPH 라디칼 소거활성 측정
    6. 순무김치의 total flavonoid 함량 측정
    7. 암세포 배양
    8. 암세포성장 및 성장 억제율 측정 (MTT assay 실험)
    9. RT-qPCR을 이용한 HT-29 세포 내 mRNA 발현 측정실험
    10. 통계 분석
결과 및 고찰
    1. 숙성기간에 따른 김치의 연도, pH, 및 산도 변화
    2. 순무김치의 DPPH 라디칼 소거능 변화
    3. 순무김치의 총 플라보노이드 함량 측정
    4. 순무김치 추출물의 농도별 HT-29 세포 성장 억제능
    5. RT-qPCR을 이용한 HT-29 세포 내 mRNA 발현 측정(Apoptosis 유전자 발현분석)
    6. RT-qPCR을 이용한 HT-29 세포 내 mRNA 발현 측정(cell cycle 관련 유전자 분석)
요약 및 결론
References

• 권국원(호서대학교 벤처대학원 융합공학과 박사과정 학생, 호서대학교 보건산업연구소 연구원) | Kook Won Kwon (Ph.D. Student, Dept. of Convergence Technology, Graduate School of Venture, Hoseo University, Seoul 06724, Korea / Researcher, Institute of Health Industry, Hoseo University, Seoul 06724, Korea)
• 강순아(호서대학교 벤처대학원 융합공학과 박사과정 학생,호서대학교 벤처대학원 융합공학과 교수, 호서대학교 보건산업연구소 소장) | Soon Ah Kang (Ph.D. Student, Dept. of Convergence Technology, Graduate School of Venture, Hoseo University, Seoul 06724, Korea/Professor, Dept. of Convergence Technology, Graduate School of Venture, Hoseo University, Seoul 06724, Korea/ Director, Institute of Health Industry, Hoseo University, Seoul 06724, Korea) Corresponding author