본 연구에서는 소비자의 안전과 관련하여 일부 한국식품에서의 벤조산과 소르빈산의 함량과 안전소비량을 평가하고자 하였다. 몇 개 브랜드의 과일 쥬스, 요거트, 치즈, 말린 과일류, 잼, 마가린을 대전의 시장에서 구입하여 HPLC로 분석하였다. column을 사용하여 chromatogmphic separation을 얻었으며 35:65 비율의 methanol acetate buffer(PH 4.4)로 벤조산과 소르빈산을 초기 mobile phase에서
Benzoic acid (BA) and sorbic acid (SA) are the preservatives most commonly used in food. Although BA and SA are generally safe, some previous studies have shown that consumption of excessive amounts of these food additives can be a health hazard. The aim of this study was to determine the amounts of BA and SA in processed foods in Korea. Different brands of fruit juice, yogurt, cheese, dried fruits, jam, and margarine were purchased at a local market in Daejeon, Korea. Samples were analyzed by high-performance liquid chromatography (HPLC) using a UV detector. Chromatographic separation was achieved with a C18 column. Methanol acetate buffer (pH 4.4) at a 35:65 v/v ratio was used as the initial mobile phase to elute BA and SA. The detector wavelength was set at 254 nm. The average test results observed for BA concentrations in fruit juice, yogurt, cheese, dried fruits, jam, and margarine were , , , , , and , respectively. Average concentrations of SA in these samples were , , , , , and , respectively. Thus, the average levels of BA and SA in the studied food items were lower than the KFDA-permitted limits. Moreover, the estimated daily intake of both BA and SA by a typical consumer were below the maximum recommended daily values.