There has been increased interest in lignans due to their potential effect in reducing the risk of developing several diseases. To evaluate lignan contents, sensitive and accurate methods should be developed for their quantification in food. The present study aimed to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5 lignans: lariciresinol (Lar), matairesinol (Mat), pinoresinol (Pin), secoisolariciresinol (Seco), and syringaresinol (Syr). The validation included selectivity, linearity, recovery, accuracy, and precision. The method was proved to be specific, with a linear response (R2≥0.99). The limits of detection were 0.040~0.765 μg/100 g and the limits of quantification were 0.114~1.532 μg/100 g. Recoveries were 90.588~109.053% for black sesame powder. Relative standard deviations of repeatability and reproducibility were below 5%. Total lignan contents of roasted coffee bean, oat, and blacksoy bean were 105.702 μg/100 g, 78.965 μg/100 g, and 165.521 μg/100 g, respectively. These results showed that LC-MS/MS analysis would be effective in producing acceptable sensitivity, accuracy, and precision in five lignan analyses.

Abstract
서 론
재료 및 방법
    1. 재료 및 시약
    2. 리그난 추출 및 전처리
    3. 기기분석 조건
    4. 표준용액 조제 및 검량선 작성
    5. 분석방법검증
결과 및 고찰
    1. 선택성 및 직선성
    2. 정량한계 및 검출한계
    3. 정밀성
    4. 정확성
    5. 종자류 식품에 함유된 리그난 함량
요약 및 결론
감사의 글
References

• 김윤정(경성대학교 식품생명공학과 대학원생) | Yoonjeong Kim (Graduate Student, Dept. of Food Science & Biotechnology, Kyungsung University, Busan 48434, Korea)
• 편지예(경성대학교 식품생명공학과 대학원생) | Jiye Pyeon (Graduate Student, Dept. of Food Science & Biotechnology, Kyungsung University, Busan 48434, Korea)
• 백인환(경성대학교 약학과 부교수) | In-hwan Baek (Associate Professor, College of Pharmacy, Kyungsung University, Busan 48434, Korea)
• 김영화(경성대학교 식품생명공학과 부교수) | Younghwa Kim (Associate Professor, Dept. of Food Science & Biotechnology, Kyungsung University, Busan 48434, Korea) Corresponding Author