This study was conducted to obtain basic information for the use of the ATP fluorescence detection method in consideration of the most common and frequent contamination situation that occurs in laboratories dealing with fire blight causing bacterium, Erwinia amylovora. ATP luminescence measurements (Relative Light Unit, RLU) were tested against these pathogen cells (CFU/cm2) which were artificially introduced on the disinfected surface of a bench floor of a biosafety cabinet (Class 2 Type A1), on a part of the disinfected surface of a lab experimental bench, on a part of the disinfected floor, and on a part of the disinfected floor of an acryl chamber for bioaerosol studies in a biosafety laboratory (BSL 2 class) using two different ATP bioluminometers. RLU values were not much increased with the bacterial cells from 2.15 × 102/cm2 to 2.15 × 106/cm2. RLU values varied among the four different surfaces tested. RLU values measured from the same number of bacterial cells differed little between the two different ATP bioluminometers used for this study. RLU values obtained from bacterial cells higher than 2.15 × 107/cm2 indicated the presence of bacterial contamination on the four different surfaces tested. The R2 values obtained based on the correlation data for the RLU values in response to different E. amylovora cell numbers (CFU/ cm2) on the surfaces of the four test spots ranged from 0.9827 to 0.9999.

Abstract
1. 서 론
2. 재료 및 방법
    2.1 세균 균주 및 배양
    2.2. 실험실 내 표면 오염 주기
    2.3 ATP 발광검출 분석
    2.4 통계 분석
3. 결과 및 고찰
4. 결 론
감사의 글
References

• 김예인(단국대학교 생명과학부 미생물학전공) | Ye In Kim (Department of Microbiology, Dankook University, Cheonan 31116, Korea)
• 조준우(단국대학교 생명과학부 미생물학전공) | Jun Woo Cho (Department of Microbiology, Dankook University, Cheonan 31116, Korea)
• 강민영(단국대학교 생명과학부 미생물학전공) | Min Yeong Kang (Department of Microbiology, Dankook University, Cheonan 31116, Korea)
• 김성환(단국대학교 생명과학부 미생물학전공) | Seong Hwan Kim (Department of Microbiology, Dankook University, Cheonan 31116, Korea) Corresponding Author