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First report of Amphidinium fijiense (Dinophyceae) from the intertidal zone of a sandy beach of Jeju Island, Korea KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/419459
A strain of Amphidinium species was established from samples collected from the intertidal zone of a sandy beach of Jeju Island, Korea. Its cells were 13.0-15.0 μm in length and 10.0-13.0 μm in width. Its cell shape was round or oval and dorsoventrally flat. A pyrenoid was located in the center of the cell and a nucleus was posteriorly located. Its epicone was small and left-deflecting. Its cingulum had V-shape on the ventral side, forming a ventral ridge and extending to the sulcus. Polygonal amphiesmal vesicles and ring-shaped body scales not described previous were observed on the surface of the cell. Its morphological features were consistent with those of previously described Amphidinium fijiense. Phylogeny based on ITS region and LSU rDNA sequences revealed that this Amphidinium isolate was clearly clustered with other A. fijiense strains, but separated from other Amphidinium species. These results indicate that this Amphidinium isolate is A. fijiense. This study reports its presence for the first time in the intertidal zone of a sandy beach of Jeju Island, Korea.
INTRODUCTION
MATERIALS AND METHODS
1. Sample collection and establishment ofstrain culture
2. Morphological observation
3. DNA extraction, PCR and sequencing
4. BLAST search and phylogenetic analysis
RESULTS AND DISCUSSION
1. Morphological features of the Korean isolateof Amphidinium fijiense
2. Checklist of the Amphidinium sensu strictospecies
3. Key to the species ofAmphidinium sensu stricto
4. Phylogenetic position of the KoreanAmphidinium fijiense
ACKNOWLEDGEMENTS
REFERENCES
