C. sakazakii ATCC 12868, 29004, 29544를 이용하여 저온 저장 중의 변화를 살펴보고 저온과 냉/해동, 저온과 산, 저온에서 starvation한 것과 냉/해동의 교차저항에 대해 알아보았다. C. sakazakii를 에서 10일간 저장하였을 때 모든 균주들에서 1 log CFU/mL의 사멸을 보였다. C. sakazakii를 에서 배양한 결과, C. sakazakii ATCC 12868, 29004는 각각 7일째, 5일째

Cronobacter sakazakii has been isolated from a wide range of environmental sources and from several foods of animal and plant origin. The objective of this study was to determine the resistance of C. sakazakii (ATCC 12868, ATCC 29004, and ATCC 29544) in cold, cold-freeze thaw, cold-acid, and cold starvation-freeze thaw stress. The number of C. sakazakii decreased to 1 log CFU/mL at (cold storage) for 10 days. When C. sakazakii was cultivated at a low temperature (), the population of C sakazakii ATCC 12868 and 29004 increased to CFU/mL, and the population of C. sakazakii ATCC 29544 increased to CFU/mL. For C. sakazakii ATCC 12868 and 29004, the cold-adapted cells ( 24 hr) decreased by 4 log CFU/mL, and the low-temperature-cultivated cells () decreased by 0.5 log CFU/mL. In this study, low-temperature cultivation enhanced the freeze-thaw cross-resistance due to the metabolic changes in the cells. Cold stress ( 48 hr, cultivation) enhanced the cold-acid cross-resistance. The cold-starved cells in the sterilized 0.1% peptone water enhanced the freeze-thaw cross-resistance with significant differences (p

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