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Production and characterization of two monoclonal antibodies against canine parvovirus 2 KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

The canine parvovirus (CPV) causes clinical signs, such as severe enteritis, dehydration, diarrhea, vomiting, leukopenia, and hair loss, which may lead to death. Vaccination is still the most important approach, as no specific treatment exists to prevent CPV. Monoclonal antibodies are valuable tools to study the pathogenic mechanisms of CPV and develop effective diagnostic reagents and pharmaceuticals. In this study, two monoclonal antibodies (MAbs) against CPV-2a were obtained through hybridoma technology by fusing myeloma cells and B cells from the spleens of mice immunized with CPV type 2a (CPV-2a). Two MAbs (CPV-330 and CPV-620) were studied on the reactivity of vaccine (CPV-2a) and field strains (CPV-new 2a, -2b, and -2c) by indirect immunofluorescence (IFA), hemagglutination inhibition test (HI), virus neutralization test (VN), and inhibition of virus growth test. Two MAbs showed similar antibody titers for HI and VN. On the other hand, CPV-330 inhibited the viral replication in Crandell-Rees Feline Kidney (CRFK) cells better than CPV-620. These CPV MAbs may provide valuable biological reagents to study the CPV pathogenic mechanisms and work as therapeutic antibodies.

목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
    Cells & Viruses
    Virus titration
    Generation of MAbs against CPV-2a
    Hemagglutination assay (HA) and Hemagglutinationinhibition test (HI)
    Immunofluorescence assay (IFA)
    Virus neutralization test (VN)
    Effect of MAbs on viral infection
RESULTS
    Generation and characterization of MAbs
    Antiviral effects of two MAbs
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
저자
  • Min-ji Kim(Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon)
  • Seong-In Lim(Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon)
  • In-Ohk Ouh(Korea Disease Control and Prevention Agency)
  • Min Ji Kim(Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon)
  • MiJung Kwon(Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon)
  • Soo Dong Cho(Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon)
  • Bang-hun Hyun(Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon)
  • Yoon-Hee Lee(Viral Disease Division, Animal and Plant Quarantine Agency, Gimcheon) Corresponding Author