The epidemiological associations between poultry farm biosecurity measures and the 2016/18 highly pathogenic avian influenza (HPAI) epidemics were evaluated using a multivariate logistic model. In the model, 11 biosecurity measures were used as independent variables in the model: a security fence to keep wild birds out of the farm, a security gate on the farm, a farm signboard, number of footbaths for disinfecting footwear, number of anterooms, U-shaped disinfection farm gate, a tunnel-shaped disinfection farm gate, a high-pressure disinfectant fogging farm gate, disinfectant booth for farm workers and visitors, high-pressure disinfectant sprayer in the farm, and personnel disinfectant sprayer in the farm. Two hundred and eighty-eight poultry farms (144 HPAI-confirmed and 144 non-confirmed) were used as the dependent variable. The numbers of footbaths and anterooms were converted to a categorical measurement format using a general additive model. The likelihood of an HPAI outbreak in a poultry farm with a fence to prevent contact between wild birds and domestic fowl was less than that of farms without a fence (OR: 0.54, P value: 0.01). The Akaike information criterion score of the multivariate model (370.91) was less than that of the univariate logistic model for each biosecurity measure. From an HPAI control perspective, it is recommended for poultry farmers to construct a wild bird-proof fence to decrease the HPAI outbreak risk.