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The SARS-CoV-2 Delta variant spike protein facilitates cell–cell fusion KCI 등재

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  • URLhttps://db.koreascholar.com/Article/Detail/419871
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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein mediates virus entry by binding to the host cell receptor, human angiotensin converting enzyme 2 (hACE2), and catalyzing virus–host membrane fusion. The S protein also mediates cell–cell fusion, potentially allowing the virus to spread virion-independently. Here, we compared the fusogenicity of SARS-CoV-2 variant S proteins using a cell–cell fusion assay. In cells overexpressing hACE2, cell–cell fusion ability of all tested SARS-CoV-2 variants was similar to that of the Wuhan-Hu-1 strain. However, in cells with endogenous hACE2, SARS-CoV-2 variants, especially the Delta variant, stimulated significantly greater cell–cell fusion than the original strain. Our results showed that the Delta variant S protein is highly fusogenic and can spread rapidly by utilizing small amounts of hACE2.

목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
    Cells
    Plasmids
    Cell-cell fusion assay
    Western blot
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
저자
  • Jinman Kim(Laboratory of Veterinary Public Health, College of Veterinary Medicine, Chungnam National University)
  • Seokin Kim(Laboratory of Veterinary Public Health, College of Veterinary Medicine, Chungnam National University)
  • Jung-Eun Park(Laboratory of Veterinary Public Health, College of Veterinary Medicine, Chungnam National University, Research Institute of Veterinary Medicine, Chungnam National University) Corresponding Author