Chlorine dioxide (ClO2) has recently emerged as an ideal disinfectant and has shown a wide range of antimicrobial activities in various pathogenic microorganisms. In this study, the virucidal effect of ClO2 at low concentration (0.02 ppm) and higher concentration (0.06 – 0.09 ppm) against Adenovirus and Herpesvirus was evaluated based on the NF T 72-281 and ASTM 1053-11 standard methods at different exposure times. The virus suspension was dried onto the carrier and then exposed to gaseous ClO2 (gClO2) at 22 ± 2∘C. For Adenovirus, exposure at a low concentration of ClO2 at the middle height resulted in the average log10 reduction of 0.95, 2.65, and 5.30 after 1, 3, and 6 h post-exposure (pe), respectively. Moreover, more than 4-log10 reduction was achieved at 4 and 6 h pe with higher concentrations of ClO2. On the other hand, the antiviral activity of gClO2 at the middle height was also effective against Herpesvirus. In particular, at 1 h pe, a less than 4-log10 reduction was observed at all examined concentrations of ClO2, whereas exposure for 3 and 6 h (with low concentration) or 2 h (with higher concentration) inactivated completely viruses attached to the carrier. These results suggested that ClO2 fumigation is a potential alternative method for disinfecting healthcare facilities, high-containment laboratories, and households with a safe concentration for human health.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Cell culture and virus stocks preparation
    Carrier preparation and gClO2 fumigation
    Viruses detachment from the carrier and endpointdilution assay
RESULTS
    Virucidal effects of gClO2 against Adenovirus
    Virucidal effects of gClO2 against Herpesvirus
DISCUSSION
ACKNOWLEDGEMENTS
CONFLICT OF INTEREST STATEMENT
REFERENCES

• Tran Xuan Ngoc Huy(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Trang Thi Nguyen(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Alisha Wehdnesday Bernardo Reyes(Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College)
• Heejin Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• WonGi Min(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Hu Jang Lee(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
• Suk Kim(Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Corresponding Author