홑파래로부터 황산기를 함유한 다당체를 크로마토그래피로 분리정제하여 rhamnan sulfate가 항보체 활성화에 미치는 영향을 조사하였다. 항보체 활성능력은 F-4-3 획분을 비교군으로 Heparin H-180, Dextran과 비교해 결과 비교군보다 높았고, C4a와 C3a의 convertase의 형성과 기능을 F-4-3 획분이 억제하였다. 이러한 보체 활성화 양식은 classical pathway 및 alternative pathway로도 경유함을 알 수 있었다.

The anti-compliment activity of hemolytic complementary assay(TCH_50) of rhamnan sulfate fraction obtained from water extracts of Monostroma nitidum was investigated Rhamnan sulfate Fraction, F-4-3 fraction appeared relatively strong anti-complementary activity which decreased TCH_50 over 60% than that comparison with control, and F-4-3 considerably inhibited ACH50 F-4-3 inhibited formation of the classical pathway C3 convertase or C4 cleavage. The results also indicatd the mode of complement activation by F-4-3 fraction shows not only the classical pathway but also the alternative pathway.

• 이혜정 | Hei Jeung Lee
• 박희옥 | Hee Ok Park
• 김유경 | Yu Kyoeng Kim