본 실험에서는 GI활성이 높은 alkalophilic Streptomyces sp.를 사용하여 2% k-carrageenan에 균체를 고정화한 후 고정화 균체의 효소학적 특성을 살펴보았다. pH stability는 pH가 7.5∼8.5에서 GI활성이 가장 높았으며, reaction temperature는 70℃, Co^2+는 10 exp (-3)M, Mg^2+는 10 exp (-3)M일 때 GI 활성이 가장 높은 것으로 나타났다.

The whole cells of alkalophilic Streptomyces sp. B-2 which produce glucose isomerase was immobilized by entrapment method for the effective production of high fructose syrup. The highest immobilized activity was achieved when the enzyme was bound to 2% k-carrageenan. Immobilized glucose isomerase the pH optimum was about pH 7.5∼8.5. Immobilization of alkalophilic Streptomyces sp. B-2 on 2% k-carrageenan at 70℃ showed an increase in glucose isomerase activity. GI activity of immobilized cells was maximum Co^2+ concentration10 exp (-3)M, Mg^2+ concentration 10 exp (-3)M.

• 송근섭 | Geun Seoub Song
• 한상배 | Sang Bae Han
• 최동성 | Dong Seong Choi