This descriptive correlational study describes the relationship between collaboration among health care professions and nurses’ organizational commitment in the operating room. A cross sectional survey of nurses (N = 142) was conducted in March 2020. The participants were nurses with more than one year work experience in operating rooms at three university hospitals in Seoul and Gyeonggi-do. Collaboration among nurses was measured using the Nurse–Nurse Collaboration Scale, while collaboration between nurses and physicians was measured by the Nurse-Physician Collaboration Scale (NPCS). All analyses were conducted using the IBM SPSS Statistics, version 23.0 with independent t-test, one-way ANOVA, Scheffé test, Pearson's Correlation, and multiple regression. The results were as follows : The collaboration among operating room nurses was scored with an average of 2.87 out of a total of 4. Collaboration between operating room nurses to physician scored 3.47 average out of 5 total. Organizational commitment scored 3.24 average out of 5 total. The factors influencing the organizational commitment of nurses in operating rooms include collaboration among nurses and effective communication, as well as collaboration between nurses and physicians for decision-making regarding treatment and nursing care. The explanatory amount of general characteristic, nurse-physician collaboration, and nurse-nurse collaboration variables was 33%, 15%, 13% respectively. Based on these findings, to enhance collaboration among operating room nurses, there is a significant need for systematic education on communication skills and decision-making competencies, continuous research, and organizational efforts.