Cordycepin (3'-deoxyadenosine) is a nucleoside analog known for its diverse range of biological activities. This study investigated the effect of different types of sawdust on the production of the bioactive compound cordycepin. The results of the study showed that different types of wood sawdust affected the biosynthesis of cordycepin and a significant increase was observed when the conventional SDB medium was replaced with 1% NaOH treated pine sawdust. To optimize cordycepin production from Paecilomyces tenuipes in a medium containing 1% NaOH-pretreated pine sawdust, we employed Response Surface Methodology (RSM) in its Box-Behnken design (BBD) canonical form. The optimal conditions were determined as follows: a particle size of 109.5111-mesh (140 m) for 1% NaOH-pretreated pine sawdust, an input weight of 21.1679 g/L, and an incubation time of 73.8423 hours. According to our model, this combination is expected to yield a maximum cordycepin content of 896.1428 g/mL. Experimental validation of this prediction was performed using the suggested optimal conditions, resulting in an average cordycepin content of 922.6771 g/mL across three replicates, thus confirming the model's accuracy.

INTRODUCTION
MATERIALS AND METHODS
    Preparation of wood sawdust
    Effects of the species of plants used for woodsawdust on cordycepin content of P. tenuipes
    Pretreatment of wood sawdust
    Effect of pretreated wood sawdust for cordycepincontent in P. tenuipes on submerged culture
    Experimental design using response surfacemethodology (RSM)
    Determination of cordycepin
    Statistical Analyses
RESULTS AND DISCUSSION
    Effect of Various Sawdust Media on CordycepinContent
    Effect of Pretreatment of Sawdust on CordycepinContent
    Effect of Particle Size and Input Weight of 1%NaOH-pretreated Pine Sawdust on CordycepinContent
    Incubation Time of C. militaris for CordycepinProduction in Medium Containing 1% NaOHpretreatedPine Sawdust
    Optimization of Cordycepin Production in P.tenuipes Cultivation Medium Containing 1% NaOHpretreatedPine Sawdust Using RSM Based on BBD
ACKNOWLEDGEMENTS

• Si Young Ha(Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea)
• Ji Young Jung(Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea)
• Jae-Kyung Yang(Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea) Corresponding author