Ipomoea aquatic is a leafy vegetable of the Convolvulaceae family, and is a tropical plant widely inhabiting southern China and Southeast Asia, and is widely known as Morning Glory in the West. In this study, the anti-inflammatory effects of ethyl acetate extract from Ipomoea aquatic extracts (IAE) were tested against lipopolysaccharide (LPS)-induced activation microglia BV2 cells. The production of nitric oxide (NO) and cell viability were measured using the Griess reagent and MTT assay, respectively. Inflammatory cytokine [interleukin (IL)-6, tumor necrosis factor (TNF)-, and interleukin-1 (IL-1)] were detected qPCR in LPS induced BV-2 cells. Subsequently, nuclear factor (NF)-B, mitogen-activated protein kinases (MAPKs), and nuclear factor erythroid-2-related factor 2 (Nrf2) were analyzed through western blot analyses and immunofluorescence. Ipomoea aquatic down-regulated of inflammatory markers and up-regulated anti-inflammatory and anti-oxidants in BV2 cells.

I. 서 론
II. 연구 내용 및 방법
    1. 재료 및 방법
    2. Ipomoea aquatic extracts (IAE) 추출물 제조
    3. 세포배양
    4. 세포 생존율 측정
    5. Nitric Oxide (NO) 농도 측정
    6. 염증조절 바이오마커 단백질 발현
    7. 염증사이토카인 mRNA 발현량 분석
    8. Immunofluorescense (IFC) 분석
    9. 통계분석
III. 결과 및 고찰
    1. IAE의 세포 생존율와 NO 생성 억제 효능
    2. IAE의 염증성 매개인자의 생성에 미치는 영향
    3. IAE가 염증성 cytokine 생성에 미치는 영향
    4. IAE의 MAPKs와 NF-kβ 신호전달 경로에 미치는 영향
    5. IAE가 Nrf2 신호전달 경로에 미치는 영향
IV. 요약 및 결론
감사의 글
Conflict of Interest

• 최지원(한국식품연구원) | Jiwon Choi (Korea Food Research Institute)
• 최상윤(한국식품연구원) | Sang Yoon Choi (Korea Food Research Institute)
• 허진영(한국식품연구원, 한국과학기술연합대학원 대학교) | Jinyoung Hur (Korea Food Research Institute, University of Science Technology (UST)) Corresponding Author