To develop a functional probiotic that inhibits gingipain, a major virulence factor of Porphyromonas gingivlais (P. gingivalis), we screened over 30 probiotic strains for their ability to inhibit gingipian activity. We investigated the inhibition of expression of gingipain genes kgp, rgpA, and rgpB as well as gingipain activity, using freeze dried cell-free supernatants of Weisiella cibaria SPM402 (WC402) and Lactobacillus paracasei SMP412 (LP412), both of which demonstrated antibacterial activity against P. gingivalis. Thus, it was verified that kgp expression was reduced by approximately 0.71±0.02 folds and rgpB expression was reduced by approximately 0.71±0.14 folds at a concentration of WC402 10 mg/mL. Meanwhile, at the same concentration of 10 mg/mL of LP412, kgp expression was reduced by approximately 0.19±0.08 folds, rgpA expression was reduced by approximately 0.09±0.02 folds, and rgpB expression was reduced by approximately 0.24±0.03 folds. At a concentration of 10 mg/mL, Kgp activity was inhibited by approximately 78.65±3.58% (cell associated gingipain, CAG), 82.45±1.22% (cell-free gingipain, CFG) by WC402 and 80.71±2.11% (CAG), and 85.81±0.05% (CFG) by LP412 respectively. Rgp activity was also effectively inhibited by approximately 78.6±1.01% (CAG), 86.78±0.47% (CFG) and 82.93±1.26% (CAG), 88.81±0.36% (CFG) by WC402 and LP412 respectively. Based on these results, W. cibaria SPM402 and L. paracasei SPM412 can be regarded as functional probiotics with the ability to inhibit gingipain activity and exhibit antibacterial effects against P. gingivalis.

Materials and Methods
    사용균주 및 배양
    WC402와 LP412의 P. gingivalis 에 대한 항균력 측정
    WC402와 LP412에 의한 gingipain 유전자 발현 억제
    WC402와 LP412에 의한 gingipain 효소활성 억제
    통계분석
Results and Discussion
    WC402와 LP412의 P. gingivalis에 대한 항균활성
    Gingipain 유전자 발현에 대한 WC402와 LP412의 영향
    Gingipain 효소 활성에 대한 WC402와 LP412의 영향
국문요약
Acknowledgement
Conflict of interests
ORCID

• 서채현(삼육대학교 약학대학) | Chae Hyeon Seo (College of Pharmacy, Sahmyook University, Seoul, Korea)
• 강소원(삼육대학교 약학대학) | Kang SoWon (College of Pharmacy, Sahmyook University, Seoul, Korea)
• 최성숙(삼육대학교 약학대학) | Sungsook Choi (College of Pharmacy, Sahmyook University, Seoul, Korea) Corresponding author