The box tree moth, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae), is a native species in East Asia specialized in box trees (Buxus sp.) but has recently invaded most European countries. Due to the absence of its natural enemies in Europe, the economic impact is serious in most countries. In order to find parasitoids as biological control agents in Korea, C. perspectalis larvae were collected from ornamental and native box trees from various regions of the country from 2022 to 2023. While rearing them in the insectary, several parasitoids emerged from the larvae. We found at least six larval parasitoids, including five wasp species and one fly species. Molecular diagnosis using the nucleotide sequences of the mitochondrial cytochrome oxidase subunit I (COI) showed that most samples were 90–97% identical to the data in the NCBI database. Further studies are required to determine biological and parasitic characteristics, such as developmental rate, fecundity, host specificity, and parasitic rate, to clarify the suitability of parasitoids as biological control agents.

• Soo-Hyun Kim(Department of Applied Biosciences, Kyungpook National University, Department of Plant Protection and Quarantine, Kyungpook National University) Corresponding author
• Eunji Lim(Dreaming Mariposas Workshop, Suwon, Korea)
• Hwal-Su Hwang(Department of Applied Biosciences, Kyungpook National University, Department of Plant Protection and Quarantine, Kyungpook National University)
• Sehyeon Lim(Department of Applied Biosciences, Kyungpook National University, Department of Plant Protection and Quarantine, Kyungpook National University)
• Ilgoo Kang(Department of Entomology, Kyungpook National University, Sangju, Korea)
• Kyeong-Yeoll Lee(Department of Applied Biosciences, Kyungpook National University, Department of Plant Protection and Quarantine, Kyungpook National University, Department of Plant Medicine, Kyungpook National University, Daegu)