Airborne bacteria in mushroom growing environments are a potential risk of contamination in commercial mushroom production. Controlling contamination in mushroom farms requires understanding the bacterial ecology in the cultivation environment. This study was conducted to investigate the concentration and species diversity of floating bacteria in a thermophilic mushroom cultivation room. Temperature, humidity, temperature, humidity, and bacterial concentration measurements were performed in April and May 2022 for a Pleurotus ostreatus cultivation house, in July and August 2023 for a Pleurotus sajor-caju and a Agaricus blazei cultivation house, and in June, July and August 2023 for a Pleurotus pulmonarius, Pleurotus sajor-caju and Calocybe indica cultivation house. The airborne bacterial concentration was 5.27 × 103~105 CFU/m3, 3.81 × 102 ~1.37 × 103 CFU/m3, and 2.55 × 102 ~1.37 × 102 CFU/m3 in the three cultivation houses, respectively. A total of 23 genera and 37 species of airborne bacteria were isolated from the three mushroom cultivation houses. 12 genera and 18 species were identified from P. ostreatus cultivation house. Furthermore, 4 genera and 4 species were found from A. blazei and C. indica cultivation house. In addition, 11 genera and 18 species were isolated from P. pulmonarius, P. sajor-caju and C. indica cultivation house. Among the bacteria isolated, the Bacilli class was the most common, followed by Gammaproteobacteria. Among the 37 bacterial species, it was determined that Bacillus cereus, B. licheniformis, Cedecea neteri, Exiguobacterium acetylicum and Raoultella terrigena could negatively affect humans or foodstuff. Cedecea neteri is also known to cause diseases among mushrooms.

1. 서 론
2. 재료 및 방법
    2.1 버섯 재배사 온도, 습도 및 부유세균 측정
    2.2 세균 분리 및 분자유전학적 분석
    2.3 분자계통분류학적 분석
3. 결과 및 고찰
    3.1 버섯 재배사 실내 온도 및 습도 조사
    3.2 버섯 재배사 실내 공기 중 부유세균 농도
    3.3 부유세균의 동정 및 특성
4. 결 론
감사의 글
References

• 윤나영(단국대학교 바이오융합대학 미생물학전공) | Na Young Yoon (Department of Microbiology, College of Life Science and Biotechnology, Dankook University, Cheonan 31116, Korea)
• 정인희(단국대학교 바이오융합대학 미생물학전공) | In Hee Jung (Department of Microbiology, College of Life Science and Biotechnology, Dankook University, Cheonan 31116, Korea)
• 김예인(단국대학교 바이오융합대학 미생물학전공) | Ye In Kim (Department of Microbiology, College of Life Science and Biotechnology, Dankook University, Cheonan 31116, Korea)
• 김성환(단국대학교 바이오융합대학 미생물학전공) | Seong Hwan Kim (Department of Microbiology, College of Life Science and Biotechnology, Dankook University, Cheonan 31116, Korea) Corresponding author
• 박혜성(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Hye Sung Park (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong 369-873, Korea)