The present study was carried out to investigate the concentration and species diversity of airborne fungi in thermophilic mushroom cultivation houses. Fungal concentration measurements were performed in April and May 2022 for a Pleurotus ostreatus cultivation house, in July and August 2023 for a Pleurotus sajor-caju and Agaricus blazei cultivation house, and in June, July and August 2023 for a Pleurotus pulmonarius, Pleurotus sajor-caju and Calocybe indica cultivation house. The airborne fungal concentration was 2.95 × 102 CFU/m3~105CFU/m3, above 105CFU/m3, and 1.12 × 103 CFU/m3~ 9.17 × 103 CFU/m3 in the three cultivation houses, respectively. A total of 8 genera and 22 species of airborne fungi were isolated from three mushroom cultivation houses. 5 genera and 7 species were identified from P. ostreatus cultivation house. Furthermore, 4 genera 6 species were found from A. blazei and C. indica cultivation house. In addition, 5 genera and15 species were isolated from P. pulmonarius, P. sajor-caju and C. indica cultivation house. Among the fungi isolated, the class of Eurotiomycetes was the most common. Among the 22 fungal species, Aspergillus flavus, A. ochraceus A. sydowii, A. tubingensis, A. westerdijkiae, Penicillium brevicompactum, P. citrinum, and P. steckii have been reported as harmful species to mushrooms, food, and human.

Airborne bacteria in mushroom growing environments are a potential risk of contamination in commercial mushroom production. Controlling contamination in mushroom farms requires understanding the bacterial ecology in the cultivation environment. This study was conducted to investigate the concentration and species diversity of floating bacteria in a thermophilic mushroom cultivation room. Temperature, humidity, temperature, humidity, and bacterial concentration measurements were performed in April and May 2022 for a Pleurotus ostreatus cultivation house, in July and August 2023 for a Pleurotus sajor-caju and a Agaricus blazei cultivation house, and in June, July and August 2023 for a Pleurotus pulmonarius, Pleurotus sajor-caju and Calocybe indica cultivation house. The airborne bacterial concentration was 5.27 × 103~105 CFU/m3, 3.81 × 102 ~1.37 × 103 CFU/m3, and 2.55 × 102 ~1.37 × 102 CFU/m3 in the three cultivation houses, respectively. A total of 23 genera and 37 species of airborne bacteria were isolated from the three mushroom cultivation houses. 12 genera and 18 species were identified from P. ostreatus cultivation house. Furthermore, 4 genera and 4 species were found from A. blazei and C. indica cultivation house. In addition, 11 genera and 18 species were isolated from P. pulmonarius, P. sajor-caju and C. indica cultivation house. Among the bacteria isolated, the Bacilli class was the most common, followed by Gammaproteobacteria. Among the 37 bacterial species, it was determined that Bacillus cereus, B. licheniformis, Cedecea neteri, Exiguobacterium acetylicum and Raoultella terrigena could negatively affect humans or foodstuff. Cedecea neteri is also known to cause diseases among mushrooms.

휴면이 타파된 무늬둥굴레의 촉성재배와 억제재배시 생육 적정 온도 범위를 알아보았다. 촉성재배를 염두한 낮과 밤의 온도가 15/5, 20/10, 25/15, 30/20oC의 처리, 억제재배를 염두한 28/ 18, 32/22, 36/26, 40/30oC 처리에서 무늬둥굴레를 생육시켰다. 촉성재배을 위한 생육온도 실험에서 온도가 올라갈수록 맹아소요 일수, 개화소요일수, 낙화소요일수가 줄어들었다. 그러나 맹아율, 개화율, 초장, 엽수에서는 온도에 따른 유의적인 차이를 보이지 않았다. 다만 초장이 15/5oC 처리에서 상대적으로 작았다. 이 실험의 결과로 촉성재배시 20/10oC 이상의 온도를 유지하는 것 이 권장할 수 있다. 억제재배를 위한 생육온도 실험에서는 재배 온도가 높아질수록 낙화소요일수, 초장, 엽수에서 감소하였다. 그 러나 맹아소요일수, 개화소요일수, 맹아율, 개화율에서는 온도간 유의적 차이가 없었다. 초장이 40/30oC 처리에서 상대적으로 줄 어드는 현상이 나타났다. 그러므로 무늬둥굴레의 억제재배시 36/ 26oC 이하의 온도로 재배할 것을 권할 수 있다.

시판되고 있는 일반 과메기(CK), 키토산을 첨가한 과메기(KAC) 및 금박을 입힌 과메기(KOGL)에 대한 일반성분, 무기질, 비타민, 지방산 및 구성아미노산 함량을 분석하였다. CK, KAC 및 KOGL의 일반성분을 분석한 결과, 수분(26.4~30.8%), 조단백질(29.1~32.7%), 조회분(1.6~1.9%)의 함량은 큰 차이를 보이지 않았으나, KOGL의 조지방 함량은 32.2%로 CK 24.5% 및 KAC 22.9%보다 상당량 높게