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4주 동안의 옥수수 추출물 투여가 마우스 비장세포와 대식세포 생성에 미치는 영향 KCI 등재

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

This study was performed to investigate the in vitro effect of a corn water extract on immune function. Splenocyte proliferation was determined by the MTT(3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl terazolium bromide) assay after preparing asingle cell suspension. Production of macrophage-secreted interleukin(IL)-1β, IL-6, and interferon(IFN)-γ, was detected by ELISA using a cytokine assay kit. After a 48-hr incubation with mitogens(ConA or lipopolysaccharide), mice splenocyte proliferation increased with the addition of a corn water extract supplement at 10, 50, 100, 250, 500, or 1, 000㎍/㎖. Production of IL-1β, IL-6, and IFN-γ increased in treatments supplemented with the corn water extract. In an in vitro study, splenocyte proliferation increased when 50~1, 000㎕/㎖ corn water extract was added. In an ex vivo experiment, the highest production of cytokines by activated peritoneal macrophages was observed in mice orally administered 500㎎/㎏ body weight/day.

저자
  • 정혜영 | Hae Young Chung