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Transcriptome analysis and identification of SSR markers in Haemaphysalis flava females using Illumina HiSeq 4000

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  • URLhttps://db.koreascholar.com/Article/Detail/433504
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한국응용곤충학회 (Korean Society Of Applied Entomology)
초록

참진드기과(Ixodidae) 속하는 개피참진드기(Haemaphysalis flava)는 동남아시아에서 남아시아에 걸쳐 분포 하며, 다양한 질병을 매개하는 것으로 알려져 있다. 특히 중국, 일본, 한국에서 개피참진드기의 주요 매개 질병인 중증열성혈소판감소증후군(SFTS)의 감염 사례가 지속적으로 증가하는 것으로 보고되고 있다. 이 연구는 Illumina HiSeq 4000 시퀀싱을 통해 raw 데이터를 획득하고, Trinity를 기반으로 de novo assembly를 수행하여 unigene을 확보하였다. 이 결과, 총 69,822개의 unigene이 생성되었으며, 이 중 46,175개의 unigene이 PANM-DB에 annotation 되었다. 또한 KOG, GO 및 KEGG 분석을 통해 30,000개, 19,074개, 9,333개의 unigene이 annotation 되었 으며, InterProScan 결과를 통해 protein kinase, zinc finger (C2H2-type), reverse transcriptase, RNA recognition motif domain 등과 같은 세포 조절 메커니즘과 관련된 유전자가 확인되었다. RepeatMasker(v4.0.6)와 MISA(v1.0)를 사 용하여 unigene에서 SSR 마커를 확인한 결과, 총 3,480개의 SSR 마커가 확인되었으며 이 중 trinucleotide 반복이 1,907개, dinucleotide 반복이 1,274개로 확인되었다. 이러한 연구 결과는 H. flava 암컷의 유전자 및 유전자 조절 메커니즘을 이해하는데 기초 자료로서 활용 가능하며, 질병 전파 감수성에 대한 후속 연구에 유용한 정보를 제공 할 것으로 사료된다.

저자
  • Chan Eui Hong(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Department of Biology, College of Natural Sciences, Soonchunhyang University)
  • Min Kyu Sang(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Research Support Center for Bio-Bigdata Analysis and Utilization of Biological Resources, Soonchunhyang University)
  • Hongray Howrelia Patnaik(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University)
  • Jie Eun Park(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Research Support Center for Bio-Bigdata Analysis and Utilization of Biological Resources, Soonchunhyang University)
  • Dae Kwon Song(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Research Support Center for Bio-Bigdata Analysis and Utilization of Biological Resources, Soonchunhyang University)
  • Jun Yang Jeong(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Department of Biology, College of Natural Sciences, Soonchunhyang University)
  • Yong Tae Kim(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Department of Biology, College of Natural Sciences, Soonchunhyang University)
  • Hyeon Jun Shin(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Department of Biology, College of Natural Sciences, Soonchunhyang University)
  • Liu Ziwei(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Department of Biology, College of Natural Sciences, Soonchunhyang University)
  • Hee Ju Hwang(Department of Biology, College of Natural Sciences, Soonchunhyang University)
  • So Young Park(Biodiversity Research Team, Animal & Plant Research Department, Nakdonggang National Institute of Biological Resources)
  • Se Won Kang(Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Seung-Hwan Park(Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Sung-Jae Cha(Department of Molecular Microbiology & Immunology and Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States)
  • Jung Ho Ko(Police Science Institute, Korean National Police University)
  • E Hyun Shin(Research Institute, Korea Pest Control Association)
  • Hong Seog Park(Research Institute, GnC BIO Co., LTD.)
  • Yong Hun Jo(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Department of Biology, College of Natural Sciences, Soonchunhyang University)
  • Yeon Soo Han(College of Agriculture and Life Science, Chonnam National University)
  • Bharat Bhusan Patnaik(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Department of Biology, College of Natural Sciences, Soonchunhyang University, PG Department of Biosciences and Biotechnology, Fakir Mohan University, Nuapadhi, Balasore- 756089, Odisha, India)
  • Yong Seok Lee(Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Research Support Center for Bio-Bigdata Analysis and Utilization of Biological Resources, Soonchunhyang University, Department of Biology, College of Natural Sciences, Soonchunhyang University)