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Anti-Inflammatory Effect of Freeze-Dried Broccoli Sprout Powder with Antioxidant Activity in RAW264.7 Cells KCI 등재
pp.156-165
Sprout products, such as broccoli, alfalfa, and cabbage, have positive health effects. Thus far, sprout foods have attracted attention owing to their good bioavailability. In particular, young broccoli sprouts exhibit anti-inflammatory, antioxidant, and anti-cancer effects. They contain 100 times more chemoprotective substances than adult broccoli. This study examined the anti-inflammatory effects of freeze-dried young sprout broccoli (FD-YB) in vitro using RAW264.7 macrophage cells. The FDYB powder antioxidant ability test showed that the radical-scavenging activity and superoxide dismutase enzyme activity increased in a dose-dependent manner. In addition, FD-YB was not cytotoxic to RAW264.7 cells, and nitric oxide production decreased after the FD-YB treatment of lipopolysaccharide-stimulated RAW264.7 cells in a dose-dependent manner. Furthermore, FD-YB significantly decreased the expression of inflammation-related proteins (Cyclooxygenase-2, Inducible nitric oxide synthase, and Prostaglandin E Synthase 2) and cytokines (Tumor necrosis factor- and Interleukin-6). In conclusion, FD-YB can be a potential nutraceutical for preventing and regulating excessive immune responses during inflammation.
II. Materials and Methods
1. Chemicals and Reagents
2. Preparation of sample
3. Preparation of the standard solutions and samples
4. DPPH Radical scavenging activity assay
5. Superoxide dismutase (SOD) enzyme activity
6. Cell culture and LPS stimulation
7. Cell viability test
8. Detection of nitric oxide (NO)
9. Measurement of cytokines
10. Western blot
11. Statistical Analysis
III. Results and Discussion
1. Antioxidant ability of FD-YB
2. Effects of FD-YB on cell viability
3. Effects of FD-YB on NO and pro-inflammatory proteinsin RAW264.7 cells
4. Effects of FD-YB on pro-inflammatory cytokines andmediators
IV. Summary and Conclusion
Conflict of Interest
References
