Supplementing feed components is becoming increasingly difficult for various reasons, including increased shipping costs, decreased capture efficiency, and global warming. For this reason, much research has been conducted globally to find alternative protein sources. The black soldier fly (BSF; Hermetia illucens) is an important feed item for nutritional accumulation and has environmental consequences. Few studies have been conducted to determine the nutritional level of the substrate, but most of these studies have involved modifying the substrate material. The aim of this study was to determine the ideal crude protein(CP) content of a substrate for BSF rearing. The experimental treatment was separated into three CP levels (low, 13.5%; medium, 20%; high, 26.5%) in the substrate. The results showed that the BSF weight (6.46%) and protein conversion ratio (1.13%) increased as the substrate CP level increased (p<0.05). In addition, protein (14.38%) and lipid (13.12%) yields were significantly increased (p<0.05). The BSF CP level increased as the substrate CP level increased (p<0.05). CP levels in the substrate upregulated the levels of certain fatty acids, essential amino acids, and non-essential amino acids (p<0.05). In conclusion, the potential values of BSF as feed were improved as the CP level of the substrate increased; However, no difference was found between medium and high CP levels in the rearing substrate. These results suggested that the substrate CP level should be at the medium level for optimal product quality. In conclusion, BSF quality is influenced by substrate nutrient levels, necessitating the development of specialized substrates for effective rearing.

Ⅰ. 서론
Ⅱ. 재료 및 방법
    1. 실험 설계
    2. 사육 성적
    3. 영양소 함량 분석
    4. 생체 전환률 분석
    5. 지방산 함량 분석
    6. 아미노산 함량 분석
    7. 통계분석
Ⅲ. 결과 및 고찰
    1. 사육 성적
    2. 영양소 조성
    3. 생체 전환효율
    4. 지방산 조성
    5. 아미노산 조성
Ⅳ. 요약
사사
Ⅴ. 참고문헌

• 김민주(한경국립대학교 동물생명융합학부 교수, 휴머니멀응용과학연구소 교수)
• 하상훈(강원대학교 동물생명과학대학 대학원생) | Sang Hun Ha (Graduate Student, Institute of Applied Humanimal Science, Hankyong National University, Ansung 17579, Korea)
• 문준영(강원대학교 동물생명과학대학 대학원생) | Jun Young Mun (Graduate Student, Institute of Applied Humanimal Science, Hankyong National University, Ansung 17579, Korea)
• 박세린(강원대학교 동물생명과학대학 대학원생) | Se Rin Park (Graduate Student, Institute of Applied Humanimal Science, Hankyong National University, Ansung 17579, Korea)
• 박선아(강원대학교 동물생명과학대학 대학원생) | Seon Ah Park (Graduate Student, Institute of Applied Humanimal Science, Hankyong National University, Ansung 17579, Korea)
• 최소담(강원대학교 동물생명과학대학 대학원생) | So Dam Choi (Graduate Student, Institute of Applied Humanimal Science, Hankyong National University, Ansung 17579, Korea)
• 김진수(강원대학교 동물생명과학대학 교수) | Jin Soo Kim (Professor, Institute of Applied Humanimal Science, Hankyong National University, Ansung 17579, Korea) Corresponding author