Purpose: This mixed methods study aimed to investigate the anxiety and nursing satisfaction levels and experiences among users of a general health checkup center. Methods: A total of 152 participants completed a questionnaire to assess their pre-checkup anxiety and post-checkup nursing satisfaction levels. Additionally, 11 participants were individually interviewed to determine their pre-checkup anxiety and post-checkup nursing satisfaction experiences. Survey data were analyzed using SPSS software, while qualitative data were analyzed using content analysis. Results: The mean anxiety scores were 2.80±2.24 on the Visual Analog Scale for Anxiety and 44.06±9.55 on the State-Trait Anxiety Inventory (STAI) scale. Education level was a significant factor influencing the STAI scores. Participants with a college education or higher had significantly lower STAI scores(p<.005), indicating the association between higher education levels and lower STAI scores. The mean nursing satisfaction score was 36.49±8.84, with male participants reporting higher nursing satisfaction levels. The pre-health checkup anxiety experience included three themes: “contrasting expectations about checkup results,” “various emotions felt during the checkup process,” and “physical and mental reactions.” The health checkup nursing satisfaction experience included four themes: “satisfaction with nurses’ support and care,” “comfort during the checkup process,” “dissatisfaction due to nurses’ habitual responses,” and “expectations for nurses’ emotional support and communication.” Conclusion: Providing comprehensive nursing information is essential to reduce user anxiety and improve nursing satisfaction. Moreover, integrating advanced technologies, such as virtual reality, augmented reality, and metaversing, into information delivery can enhance educational effectiveness and better address the experiences and needs of checkup users.