Biomimetic study is being conducted in various fields and applied to the development of technology for the realization of a sustainable society. In this study, we analyzed the cuticular surface structure and wax layer composition of the leaves of Myriophyllum verticillatum and Azolla imbricata to investigate the antifouling characteristics. Field emission scanning electron microscopy analysis (FE-SEM) and contact angle measurement revealed that the surface of M. verticillatum had an irregular and curved layered structure with non-directional filament structures and showed high hydrophobicity. On the leaves of A. imbricata, amphiphilic structures with nano-sized hydrophobic plate-like filament structures and micro-sized irregular hydrophilic spikes were observed. The dorsal surface of the A. imbricata leaves had a denser distribution of hydrophobic nano-structures compared to the ventral surface. The dorsal surface of the leaf showed high hydrophobicity, while the ventral surface showed wettability. Due to the habitat characteristics of A. imbricata, which is a floating plant, the ventral surface is constantly in contact with water, while the front surface has a hydrophobic surface. Analysis of the wax composition of plants revealed that M. verticillatum and A. imbricata were mainly composed of saturated fatty acids, ketones (2-Nonadecanone and 2-Heptadecanon), and sugar alcohols such as 1,30-Triacontanediol. These substances have high antimicrobial activity, and saturated fatty acids form stable and rough anisotropic crystalline surfaces. The hierarchical amphiphilic structure and the non-toxic stable hydrophobic surface observed in the cuticle of aquatic plants are expected to be utilized as materials for the development of sustainable antifouling technologies.

Abstract
서 론
재료 및 방 법
    1. 식물 샘플 채취 및 미세구조 분석
    2. 접촉각 (Contact Angle) 측정
    3. GC/Quadrupole-MS 분석
    4. MS-DIAL을 이용한 GC/MS 데이터 분석
결과 및 고 찰
    1. 표면 형태
    2. 접촉각 분석
    3. 성분 분석
적 요
REFERENCES

• 박정은(국립생태원 생태응용연구실 생태신기술팀) | Jeongeun Bak (Ecological Technology Research Team, Division of Ecological Applications Research, National Institute of Ecology, Seocheon 33657, Republic of Korea)
• 정선아(아주대학교 생명과학과 생태학연구실) | Seonah Jeong (Department of Biological Science, Ajou University, Suwon 16499, Republic of Korea)
• 문새로미(국립생태원 생태평가연구실 복원생태팀) | Saeromi Mun (Ecological Restoration Team, Division of Ecological Assessment, National Institute of Ecology, Seocheon 33657, Republic of Korea) Corresponding author