논문 상세보기
pp.322-332
Advanced glycation end products (AGEs) trigger various metabolic diseases and accumulate in the organs during the onset of metabolic diseases. The AGE-receptor for AGE (RAGE) interactions are strongly associated with the onset of chronic renal disease and diabetic nephropathy. This study evaluated the effects of glyoxal-lysine dimer (GOLD) and methylglyoxal-lysine dimer (MOLD) accumulation on various mechanisms in SV40 MES 13 kidney cells, which are currently unclear. GOLD and MOLD showed different effects on oxidative stress, inflammation, mitochondrial dysfunction, autophagy, and apoptosis. GOLD did not induce cytotoxicity or interact with RAGE. By contrast, MOLD significantly reduced the cell viability and interacted with RAGE. This study tested whether the RAGE interaction could cause differences in the effects of GOLD and MOLD on the mechanisms studied. GOLD did not generate oxidative stress or interact with RAGE and did not show toxicity through other mechanisms. Nevertheless, MOLD caused oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis, which are representative glucotoxicity mechanisms of AGE-RAGE interactions, and autophagy. Overall, these findings suggest that AGEs may show different toxicities in various organs. GOLD accumulation in the kidneys might not affect disease occurrence, but MOLD accumulation can promote disease.
I. 서 론
II. 연구 내용 및 방법
1. 재료 및 방법
2. 세포배양 및 세포독성 측정
3. 세포 내 Reactive Oxygen Species (ROS) 측정
4. Western blot을 통한 항산화 단백질 발현분석
5. 자가포식(Autophagy) 측정
6. 세포사멸(Apoptosis) 분석
7. 통계분석
III. 결과 및 고찰
1. 최종당화산물이 신장세포 생존률 및 RAGE단백질 발현에미치는 영향
2. 최종당화산물이 산화 스트레스에 미치는 영향
3. 최종당화산물이 염증 반응에 미치는 영향
4. 최종당화산물이 Apoptosis 및 미토콘드리아 막전위 변화에 미치는 영향
5. 최종당화산물이 자가포식(Autophagy) 유도에 미치는 영향
6. 최종당화산물이 MAPK의 활성에 미치는 영향
V. 요약 및 결론
감사의 글
Conflict of Interest
References
