Chrysanthemum coronarium L (CC)., a leafy vegetable, has various biological properties, including anti-oxidative, antiinflammatory, antiproliferative, and anti-osteoporosis effects. However, the vascular protective effects of CC remain poorly understood. In this study, we identified the vascular protective effect of CC against ferroptosis in aortic thoracic smooth muscle A7r5 cells. The vascular protective effects of CC against erastin (Era)-induced A7r5 cells were assessed by estimating cell viability, glutathione (GSH) levels, lipid peroxidation, mitochondrial morphological change, and using western blot analysis. The CC treatment effectively ameliorated Era-induced ferroptotic cytotoxicity, including cellular death. The treatment also suppressed mitochondrial morphological change in the Era-induced A7r5 cells. CC significantly regulated Era-induced abnormal mechanisms related to GSH, lipid peroxidation, cysteine/glutamate antiporter SLC7A11 (xCT), and glutathione peroxidase 4 (GPX4) protein expression in A7r5 cells. In conclusion, these findings indicate that CC shows potential as a functional food supplement, nutraceutical, or medicinal food, with protective effects with respect to vascular health by regulating ferroptosis.

Abstract
I. 서 론
II. 연구 내용 및 방법
    1. 소재 준비
    2. 세포 배양 및 세포 처리
    3. 세포 생존율(Cell viability)
    4. 지질 과산화(Lipid peroxidation)
    5. Glutathion 측정
    6. 투과 전자 현미경(TEM, Transmission electron microscope)
    7. 단백질 발현 측정(Western blot analysis)
    8. 통계처리
III. 결과 및 고찰
    1. 쑥갓 추출물의 세포 생존율 평가
    2. 쑥갓 추출물의 Era에 의해 손상된 미토콘드리아 형태 변화분석
    3. 쑥갓추출물의 Era에 의해 유도된 GSH와 지질 과산화(lipidperoxidation)의 회복 효능
    4. 쑥갓 추출물의 Era에 의해 감소한 xCT와 GPX4의 회복효능
IV. 요약 및 결론
감사의 글
Conflict of Interest
References

• 이상희(한국식품연구원) | Sang Hee Lee (Korea Food Research Institute)
• 성미정(한국식품연구원) | Mi Jeong Sung (Korea Food Research Institute) Corresponding author