Clove (Syzygium aromaticum) is a highly valued medicinal plant native to Aisa. Widely used as a spice, renowned for its medicinal properties, particularly in Ayurveda and traditional Chinese medicine. In this study, clove bud extract (CBE) was prepared at different ethanol concentrations of 50%, 80%, and 90%, respectively. The antioxidant activity of the CBE was evaluated through DPPH, polyphenol, and reducing power assays, revealing its strong antioxidant potential, with 90% ethanol being the most effective extract. HPLC analysis identified eugenol (8.7 mg/g) as the major active compound, known to possess anti-inflammatory and antioxidant properties. Given the role of oxidative stress and inflammation in atopic dermatitis (AD), the therapeutic potential of CBE was explored using a 1-chloro-2, 4-dinitrobenzene (DNCB)-induced AD mouse model. Five-week-old BALB/c mice were induced with AD by topical application of DNCB. CBE was administered topically to the affected skin (back and ear) areas for 4 weeks. The treatment of CBE significantly reduced the severity of clinical dermatitis, decreased epidermal thickness, and lowered mast cell and eosinophil infiltration in skin tissue, as observed through hematoxylin eosin staining and toluidine blue staining. The results demonstrated CBE as a promising therapeutic agent for managing AD through its regulation of skin inflammation and oxidative stress, making it a potential candidate for future treatments of inflammatory skin disorders.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Extraction process of clove bud extract (CBE)
    Measurement of total polyphenol content
    DPPH radical scavenging assay
    Reducing power assay
    Quantification of eugenol content in CBE using HPLCanalysis
    Experimental animals
    Experimental study using CBE for AD mice
    Measurement of ear thickness and organ weight
    Histopathological studies
    Statistical analysis
RESULTS
    Determination of total polyphenol contents of CBE
    Determination of radical scavenging activities of CBE
    Determination of total reducing power ability of CBE
    Determination of eugenol content in CBE using HPLCanalysis
    CBE alleviated skin symptoms in DNCB-inducedmice
    Inhibitory effects of CBE on DNCB-induced AD skinsymptoms in BALB/c mice
DISCUSSION
REFERENCES

• Most Nusrat Zahan(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Yujin Kim(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Jiwon Chung(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Donghyeon Lee(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Jong Hyuk Kim(Institutes of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea)
• II Rae Rho(Institutes of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea)
• Il-Hwa Hong(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea, Institutes of Animal Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Euikyung Kim(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea, Institutes of Animal Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Changkeun Kang(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea, Institutes of Animal Medicine, Gyeongsang National University, Jinju 52828, Korea) Corresponding author