In this study, an simultaneous LC-MS/MS multi-residue analytical method was developed and validated for the residues of six neonicotinoid insecticides (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam) in honey. Sample preparation included a combination of QuEChERS extraction kit and liquid-liquid extraction method to effectively extract pesticide components from the honey matrix and optimized analytical conditions to achieve high sensitivity and selectivity. The limits of detection (LOD) and the limits of quantitation (LOQ) were set in the range of 6-15 ng/mL and 19-44 ng/mL, respectively and the correlation coefficient (R²) was greater than 0.99, confirming good linearity. In addition, the intra-day recoveries for each pesticide were 75-104%, and the coefficient of variation (CV) was less than 20%, which met the guideline recommended by the Ministry of Food and Drug Safety. The LC-MS/MS method developed in this study is expected to be used as a multi-residue analysis method for 6 neonicotinoid pesticides in honey.

Abstract
서 론
재료 및 방법
    시약 및 기기
    기기분석
    표준용액의 제조
    시료 전처리 과정
    분석법 검증
    선택성
    직선성
    검출한계 및 정량한계
    정확도 및 정밀도
결과 및 고찰
    선택성
    직선성
    검출한계 및 정량한계
    정확도 및 정밀도
감사의 글
REFERENCES

• Su Min Nam(Department of Animal Health and Welfare, Semyung University, Chungbuk 27136, Korea)
• Jin Hyang Bae(Department of Animal Health and Welfare, Semyung University, Chungbuk 27136, Korea)
• Do Hui Kim(Department of Animal Health and Welfare, Semyung University, Chungbuk 27136, Korea)
• Hwan Goo Kang(Department of Animal Health and Welfare, Semyung University, Chungbuk 27136, Korea) Corresponding author