This study investigated benthic macroinvertebrates and fish in the Yeongsangang River weir and analyzed stomach contents of Squalidus chankaensis tsuchigae. Length distribution of individuals varied by site, with 91.3% and 72.7% falling within the 70-90 mm range in SC-U (upstream location of Seungchon weir) and SJ (location between Seungchon and Juksan weirs), respectively, and 77.5% within 70-80 mm and 100 mm range in JS-D (downstream location of Juksan weir). Chironomidae spp. (nonred type) were the most abundant food sources, comprising 46.3%, 76.9%, and 88.3% of the diet at SC-U, SJ, and JS-D, respectively. The Electivity Index (EI) indicated a strong preference for Chironomidae spp. (non-red type) (0.927) and avoidance for the red type (-0.929) and Limnodrilus gotoi (-0.995). The proportion of burrowers (BU) increased downstream, while swimmers (SW) decreased. The condition factor (K) varied by site, decreasing in SC-U and increasing in SJ and JS-D, showing a significant positive influence on the number of food sources (p<0.05). Among food sources, Chironomidae spp. and Baetidae sp. were predicted to be utilized across a broad range of lengths and weights, with strong connectivity observed among Tanypodinae sp., Hymenoptera sp., and Baetiella tuberculata.