Three strains (KCOM 2191, KCOM 2668, and KCOM 2812) of Capnocytophaga sp. isolated from a Korean population were initially classified by 16S rDNA sequence comparison. This study aimed to resolve their species-level identity using whole genome sequencing and to assess their taxonomic characteristics. Genomes of the three strains were sequenced using PacBio RS II and Illumina platforms. Average nucleotide identity (ANI) analysis was employed for species-level identification. Cellular fatty acid (CFA) compositions were determined using the MIDI/Hewlett Packard Microbial Identification System. ANI values for KCOM 2191, KCOM 2668, and KCOM 2812 were 96.43%, 96.33%, and 96.33%, respectively, compared with the type strain Capnocytophaga ochracea DSM 7271T. CFA profiling showed a predominance of iso-C15:0 (57.9%, 67.2%, and 64.9%, respectively), consistent with DSM 7271T (51.5%). These findings confirm that KCOM 2191, KCOM 2668, and KCOM 2812 are strains of C. ochracea . These strains may serve as valuable models for investigating the role of C. ochracea in oral and systemic pathogenesis.

Introduction
Materials and Methods
    1. Bacteria and bacterial culture
    2. Phylogenetic analysis based on 16S rDNA nucleotidesequences
    3. Bacterial WGS determination and species-levelidentification
    4. Morphological characterization
    5. Biochemical characterization
    6. Bacterial fatty acid composition analysis
    7. Polar lipid analysis
    8. Quinone analysis
    9. Determination of optimal growth conditions
Results
    1. 16S rDNA sequence homology and phylogeneticanalysis
    2. Three strains identified as C. ochracea through ANIand GGDC analysis
    3. Morphological characterization by SEM andstereomicroscopy
    4. Biochemical characteristics
    5. Analysis of bacterial fatty acid composition, polarlipids, and quinones
    6. Optimal growth conditions
Discussion
Funding
Conflicts of Interest
Supplementary Data
References

• Joong-Ki Kook(Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea, Oral Bacterial Pathogen Resource Specialized Bank and Korean Collection for Oral Microbiology, Chosun University, Gwangju 61452, Republic of Korea) Corresponding author
• Soon-Nang Park(Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea, Oral Bacterial Pathogen Resource Specialized Bank and Korean Collection for Oral Microbiology, Chosun University, Gwangju 61452, Republic of Korea)
• Yun Kyong Lim(Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea, Oral Bacterial Pathogen Resource Specialized Bank and Korean Collection for Oral Microbiology, Chosun University, Gwangju 61452, Republic of Korea)