간행물
International Journal of Oral Biology KCI 등재후보
- 발행기관 대한구강생물학회
- 자료유형 학술지
- 간기 계간
- ISSN 1226-7155 (Print)2287-6618 (Online)
- 수록기간 1977 ~ 2025
- 주제분류 의약학 > 치의학 의약학 분류의 다른 간행물
- 십진분류KDC 515DDC 610
권호리스트/논문검색
Vol.50 No.2 (2025년 6월) 4건
1.
2025.06
구독 인증기관 무료, 개인회원 유료
Three strains (KCOM 2191, KCOM 2668, and KCOM 2812) of Capnocytophaga sp. isolated from a Korean population were initially classified by 16S rDNA sequence comparison. This study aimed to resolve their species-level identity using whole genome sequencing and to assess their taxonomic characteristics. Genomes of the three strains were sequenced using PacBio RS II and Illumina platforms. Average nucleotide identity (ANI) analysis was employed for species-level identification. Cellular fatty acid (CFA) compositions were determined using the MIDI/Hewlett Packard Microbial Identification System. ANI values for KCOM 2191, KCOM 2668, and KCOM 2812 were 96.43%, 96.33%, and 96.33%, respectively, compared with the type strain Capnocytophaga ochracea DSM 7271T. CFA profiling showed a predominance of iso-C15:0 (57.9%, 67.2%, and 64.9%, respectively), consistent with DSM 7271T (51.5%). These findings confirm that KCOM 2191, KCOM 2668, and KCOM 2812 are strains of C. ochracea . These strains may serve as valuable models for investigating the role of C. ochracea in oral and systemic pathogenesis.
4,300원
2.
2025.06
구독 인증기관 무료, 개인회원 유료
Delayed wound healing in diabetes is aggravated by chronic inflammation and impaired keratinocyte migration. Although zinc facilitates skin wound healing, its excessive accumulation may induce cytotoxicity. This study explores the synergistic potential of zinc and the GPR39 agonist TC-G-1008 in enhancing diabetic wound repair. In vitro , cotreatment reduced lipopolysaccharide-induced pro-inflammatory cytokine expression and promoted keratinocyte migration. In vivo , a hydrogel incorporating zinc oxide nanoparticles and TC-G-1008 accelerated wound closure in diabetic mice and suppressed interleukin-1β expression. Notably, TC-G-1008 alone enhanced keratinocyte migration under diabetic conditions, highlighting its critical role in modulating inflammation. These findings support a zincsparing therapeutic approach using GPR39 activation to restore wound healing in diabetic settings.
4,200원
3.
2025.06
구독 인증기관 무료, 개인회원 유료
Periodontitis is a chronic inflammatory condition primarily triggered by bacterial infections, with periodontopathogens such as Porphyromonas gingivalis playing a pivotal role. We evaluated the antioxidant and anti-inflammatory effects of ethanol extract of Salvia plebeia R. Br. (SP-E) on human gingival fibroblasts (hTERT-hNOF) stimulated with P. gingivalis -derived lipopolysaccharide (LPS). Dried S. plebeia was extracted using 70% ethanol, yielding a 10.5% extract. Inflammation in hTERT-hNOF cells was induced using P. gingivalis LPS in conjunction with LPS-binding protein and CD14. SP-E was administered at concentrations ranging from 25 to 100 μg/mL. Antioxidant capacity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and superoxide dismutase (SOD) activity assay. Inflammatory cytokine expression and secretion were analyzed via reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results demonstrated a concentrationdependent antioxidant effect, with 62.98% radical scavenging activity observed at 200 μg/mL SP-E. In hTERT-hNOF cells, SOD activity increased from 4.88% (LPS-treated) to 45.78% with 100 μg/mL SP-E. RT-PCR analysis showed significant downregulation of interleukin (IL)-1β, IL-6, and IL-8 mRNA expression following SP-E treatment. ELISA confirmed a reduction in tumor necrosis factor (TNF)-α (312.83 → 178.22 pg/mL), IL-6 (453.97 → 170.83 pg/mL), and IL-8 (480.14 → 276.86 pg/mL) levels with 100 μg/mL SP-E. These findings suggest that SP-E may offer therapeutic potential for preventing and managing periodontal disease by mitigating oxidative stress and modulating inflammatory cytokine expression. Further studies are warranted to elucidate the underlying molecular mechanisms and validate these effects in vivo .
4,000원
4.
2025.06
구독 인증기관 무료, 개인회원 유료
Fabry disease is an X-linked lysosomal storage disorder caused by GLA mutations, leading to a deficiency in α-Galactosidase A activity and subsequent accumulation of globotriaosylceramide (Gb3). This accumulation contributes to progressive multiorgan dysfunction, with cardiovascular complications, particularly endothelial dysfunction and left ventricular hypertrophy being major drivers of disease morbidity and mortality. Although enzyme replacement therapy is currently the standard treatment, its effectiveness is limited in addressing advanced cardiovascular pathology. To better understand Fabry-associated vascular and cardiac phenotypes, an isogenic human induced pluripotent stem cell (hiPSC) model in which GLA was knocked out was developed using CRISPR/ Cas9. GLA-knockout (GLA-KO) hiPSCs were differentiated into endothelial cells (ECs) and cardiomyocytes (CMs) to evaluate disease-relevant phenotypes in vitro . GLA-KO ECs exhibited normal morphology and differentiation capacity but showed markedly impaired tube formation, high expression of inflammatory genes ICAM1, VCAM1, and SELE, and increased mitochondrial and cytoplasmic reactive oxygen species levels. GLA-KO CMs demonstrated enlarged cell size and nuclear translocation of NFATC4, consistent with hypertrophic remodeling. Together, these findings recapitulate key features of Fabry vasculopathy and cardiomyopathy in a genetically defined, human-derived system. This platform enables direct investigation of Gb3-induced oxidative and inflammatory mechanisms and provides a valuable model for the preclinical evaluation of therapeutic strategies targeting the cardiovascular manifestations of Fabry disease.
4,000원
