간행물
International Journal of Oral Biology KCI 등재
- 발행기관 대한구강생물학회
- 자료유형 학술지
- 간기 계간
- ISSN 1226-7155 (Print)2287-6618 (Online)
- 수록기간 1977 ~ 2025
- 주제분류 의약학 > 치의학 의약학 분류의 다른 간행물
- 십진분류KDC 515DDC 610
권호리스트/논문검색
Vol.50 No.4 (2025년 12월) 8건
1.
2025.12
구독 인증기관 무료, 개인회원 유료
Trigeminal neuralgia is a neuropathic pain disorder characterized by sudden, intense, and recurrent episodes of electric shock-like pain within the distribution of the trigeminal nerve. In addition to pain triggered by normally nonpainful stimuli, it may also present as spontaneous episodes. Although various causes of trigeminal neuralgia have been identified―such as neurovascular compression, demyelination, injury, and tumors―the underlying pathophysiological mechanisms remain poorly understood. Understanding these mechanisms is therefore crucial; however, the animal models used in research do not fully replicate the causes of the disorder. This review provides an overview of the animal models employed in trigeminal neuralgia research and evaluates their suitability for investigating disease mechanisms. Among these models, trigeminal root compression and demyelination models most closely reproduce the clinical etiology of trigeminal neuralgia. Developing animal models that accurately reflect the clinical pathophysiology of trigeminal neuralgia is essential for elucidating the underlying mechanisms and advancing the preclinical evaluation of new pharmacological agents and therapeutic interventions.
4,000원
2.
2025.12
구독 인증기관 무료, 개인회원 유료
The present study evaluated the antibacterial efficacy of Maclura tricuspidata root (MTroot) extracts against the cariogenic bacterium Streptococcus mutans. The chloroform (CHCl3) fraction obtained from organic solvent partitioning of the MTroot ethanol extract was subfractionated using open-column chromatography on silica gel. Among the subfractions obtained, subfraction 3 (Fra-3) obtained in 1:1 (v/v) n-hexan:ethyl acetate solvent system exhibited the most potent antibacterial activity. Accordingly, the chemical composition was analyzed using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry in positive electrospray ionization mode. In addition, the antibacterial activity of Fra-3 against S. mutans was assessed at each stage of extraction. While the minimum inhibitory concentrations (MICs) of the CHCl3, EtOAc, n-butanol, and aqueous fractions against S. mutans were above 15, 120, 120, and 120 μg/mL, respectively, the MICs of the subfractions obtained from silica gel open-column chromatography using different n-hexane:ethyl acetate ratios (3:1, 1:1, and 1:3) were 3.0, 1.5, and 25.0 μg/mL, respectively. Fra-3 was tentatively found to contain 38 major compounds, including kuwanon C, kuwanon E, cudratricusxanthone L, morusin C, cycloartocarpesin, kuwanon A, mulberrofuranol, and moracin U, which accounted for 66.7% of the total ion response. Collectively, these findings suggest that kuwanon C, kuwanon E, and cudratricusxanthone L present in MTroots may serve as novel therapeutic targets to prevent dental caries caused by S. mutans.
4,200원
3.
2025.12
구독 인증기관 무료, 개인회원 유료
Naringenin (5,7,4’-trihydroxyflavanone) is a natural bioflavonoid with numerous documented bioactivities in the central nervous system. The substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc; medullary dorsal horn) is recognized as a pivotal site for integration and modulation of orofacial nociceptive inputs. However, the potential effects of naringenin on orofacial pain responses and the contributions of GABAA receptor modulation have not yet been examined. In this study, whole-cell patch-clamp technique was applied to evaluate the effects of naringenin on the GABAA receptor responses on SG neurons in the Vc, and to assess potential sex-related differences. The GABAA receptor agonist muscimol was applied alone or with naringenin on SG neurons in the Vc that were patch-clamped with high chloride pipette solution to augment GABAA potentials. Naringenin increased both the amplitude and the area under the curve of muscimol-mediated responses in the majority (64.7%) of neurons tested (n = 17), and these effects differed by sex, suggesting that naringenin may modulate orofacial pain in a sex-dependent manner by enhancing GABAA potentials on SG neurons in the Vc. Naringenin may thus be a promising prototype compound for developing therapeutic agents against orofacial pain.
4,000원
4.
2025.12
구독 인증기관 무료, 개인회원 유료
This retrospective study evaluated the efficacy of bone regeneration following decortication and sequestrectomy combined with the application of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP-2/ACS) in 45 patients with medication-related osteonecrosis of the jaw (MRONJ). Each patient received 0.25 g of rhBMP-2/ACS at the surgical site. Facial computed tomography scans were obtained immediately postoperatively and at a six-month follow-up to assess volumetric changes in the alveolar bone. The results indicated that rhBMP-2 significantly enhanced bone regeneration. Notably, the number of residual bone walls was the only significant factor influencing the regenerative outcome (B = 19.47, standard error = 3.05, t = 6.39, p < 0.001). Four-wall defects exhibited the highest regeneration rate (85.2%), and the mandibular posterior region was the most frequently involved site (60.5%). Complications such as surgical site disruption did not significantly impact the regenerative outcomes. These findings suggest that the concomitant use of rhBMP-2 with decortication and sequestrectomy promotes bone healing, reduces recovery time, and improves treatment predictability for patients with MRONJ.
4,000원
5.
2025.12
구독 인증기관 무료, 개인회원 유료
Osteoprotegerin (OPG), a conventional potent inhibitor of osteoclastogenesis, is being increasingly recognized for its diverse roles beyond bone metabolism. However, the cell-autonomous role of OPG in regulating the differentiation and fate of mesenchymal progenitor cells (MPCs) remains to be fully elucidated. To address this issue, OPG-knockout (OPG-KO) human induced pluripotent stem cell-derived MPCs were generated using CRISPR/Cas9 technology. Transcriptomics revealed that OPG deficiency fundamentally alters the functional landscape of the MPCs, with a significant downregulation of the pathways related to the extracellular matrix (ECM), cell adhesion, and structural signaling. Specifically, the expression of numerous key ECM components was broadly attenuated in OPG-KO MPCs. Such molecular disruption functionally translated into severely impaired osteogenic potential, characterized by a marked transcriptional attenuation of osteogenic markers and reduced matrix mineralization at the cell level. Collectively, our findings demonstrate that OPG is essential for maintaining the structural integrity of the MPC niche by regulating the expression of ECM-related genes, thereby promoting osteoblast differentiation.
4,000원
6.
2025.12
구독 인증기관 무료, 개인회원 유료
Si Yeong Kim, Woo-Gyun Choi, Hoi-Soon Lim, Yu Ri Song, Pitna Kim, Hyung Keun Kim, In-Chol Kang, Jin Chung
Inflammation is a fundamental host defense mechanism against external insults; however, excessive immune activation contributes to inflammatory diseases such as periodontitis, resulting in periodontal tissue destruction and tooth loss. Interleukin-1β (IL-1β), a key pro-inflammatory cytokine, stimulates oral epithelial cells to produce interleukin-8 (IL-8), which recruits neutrophils and amplifies local inflammation. Therefore, regulation of IL-1β– induced IL-8 secretion in oral epithelial cells is critical for controlling pathological inflammatory responses. Peptidebased therapeutics have attracted increasing interest due to their specificity and biocompatibility, highlighting their potential as anti-inflammatory agents. This study investigated the anti-inflammatory effects and mechanisms of a human stromal cell–derived factor-1 (SDF-1)–derived peptide in IL-1β–stimulated oral epithelial cells. Human oral epithelial KB cells and immortalized human oral keratinocytes were treated with IL-1β in the presence or absence of SDF-1–derived peptides. IL-8 secretion was measured by enzyme-linked immunosorbent assay, and activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) pathways was examined by western blotting. IL-1β significantly increased IL-8 secretion and induced phosphorylation of NF-κB p65 and MAPKs, including extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase, and p38. Inhibition of ERK and p38 markedly reduced IL-8 expression, indicating their central roles in IL-1β signaling. Among 18 SDF-1δ–derived peptides, S12 exerted the strongest inhibitory effect, reducing IL-8 secretion and suppressing IL- 1β–induced NF-κB and MAPK phosphorylation. These results demonstrate that S12 attenuates IL-1β–driven IL-8 production by targeting key inflammatory signaling pathways, supporting its potential as a host-modulation therapeutic for periodontal disease.
4,000원
7.
2025.12
구독 인증기관 무료, 개인회원 유료
Dental caries is one of the most common infectious diseases. Although its prevalence has declined significantly in many countries, dental caries in Korea remains a public health concern. The essential process in dental caries involves demineralization of the tooth surface due to a lowered pH in dental plaque, which contains various microorganisms. Streptococcus mutans is believed to play a major role in the development of dental caries by producing high concentrations of organic acids. This study aimed to evaluate the cariogenic potential of the lytic enzyme L27, derived from Bacillus licheniformis YL-1005, isolated from Korean soil. Enzyme L27 was not cytotoxic to normal periodontal ligament cells at concentrations below 250 μg/mL and had minimal impact on the pH changes caused by S. mutans . Calcium ion release occurred slowly and progressively during the first 20 minutes, but demineralization was significantly inhibited by L27. The attachment of S. mutans to hydroxyapatite beads and the reduction in surface hardness were also significantly inhibited, although the rate of bacterial attachment decreased over time. Taken together, these findings suggest that the lytic enzyme L27 from soil-derived bacteria exhibits anticariogenic effects by preventing bacterial attachment to the enamel surface and inhibiting demineralization.
4,000원
8.
2025.12
구독 인증기관 무료, 개인회원 유료
Diabetes mellitus is a highly prevalent chronic metabolic disorder associated with an elevated risk of diabeticinduced neuropathic pain and affective disturbances. However, animal models that recapitulate events of peripheral neuropathic pain and emotional alterations remain insufficiently characterized. This study aimed to investigate the association between hindpaw mechanical allodynia and distinct affective behavioral changes in a streptozotocin (STZ)-induced diabetic mouse model. Adult male C57BL/6 mice received a single intraperitoneal STZ injection (150 mg/kg), and body weight and blood glucose levels were monitored weekly for two weeks. Mechanical sensitivity was evaluated using von Frey filaments at baseline and at one and two weeks post-injection. Affective behaviors were assessed through the elevated plus maze (EPM), open field test (OFT), tail suspension test (TST), and marble burying test (MBT). STZ-treated mice developed sustained hyperglycemia and progressive weight loss. Furthermore, they displayed hindpaw mechanical allodynia since 1st week after injection. Although anxiety-like behavioral changes in the EPM were not prominent, STZ-treated mice exhibited reduced center-zone duration and locomotion in the OFT, along with fewer buried marbles in the MBT. Conversely, immobility time in the TST remained unchanged. These findings indicate that STZ-induced diabetic mice exhibit both peripheral mechanical hypersensitivity and selective depression-like behavioral alterations that differ across test paradigms, underscoring the need for diverse behavioral assessments when investigating diabetes-related pain-depression comorbidity.
4,000원
