This study was conducted to evaluate the germination rate, chemical composition, and in vitro digestibility of sprouted barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) sprouts depending on cultivar and growth duration. Four cultivars Keunalbori1ho, Saekeumkang, Arijinheuk, and Jokyoung were tested under hydroponic and nutrient solution conditions. The germination rate was significantly higher under hydroponic conditions compared to nutrient solution treatment. Sprouts were harvested at 0, 4, 6, and 8 days for subsequent analysis. Chemical compositions, such as dry matter (DM), crude protein (CP), ether extract (EE), and crude ash (CA), were analyzed following AOAC (2005) protocols, while neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents were determined according to the method of Van Soest et al. (1991). In vitro digestibility was assessed by incubating ground samples with rumen buffer in DaisyII incubators for 48 h. At day 6 of growth, Keunalbori1ho showed the highest NDF and ADF concentrations (48.6% and 26.2%), reflecting its high structural fiber and the lowest digestibility (IVDMD 52.2%, IVNDFD 37.2%). However, it maintained consistent nutritional characteristics across all growth stages, suggesting potential as a stable forage source. In contrast, Saekeumkang exhibited the highest IVNDFD (59.1%) along with relatively low NDF (30.5%) and ADF (16.7%) values (p<0.05), indicating superior digestibility likely attributed to a simpler cell wall structure and higher NFC availability. Notably, digestibility sharply declined by day 8, implying that the optimal harvest window lies around day 6. Therefore, this study suggests that the cultivars Keunalbori1ho and Saekeumkang possess complementary strengths in terms of chemical composition and digestibility, underscoring the importance of optimizing cultivar selection and harvest timing to enhance the potential of cereal sprouts as high quality forage.