논문 상세보기
The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene derived from Agrobacterium sp. strain CP4 is known to confer herbicide resistance. It has been extensively utilized in the development of living modified organism (LMO) over the past 25 years. With increasing importation of LMO agricultural products, there is a growing need for rapid and reliable detection methods for detecting the CP4 EPSPS protein. In this study, a rapid immunoassay kit based on the lateral flow assay (LFA) principle was developed to detect herbicide-resistant crops. Using recombinant CP4 EPSPS protein developed by the National Institute of Ecology, two in-house antibodies were produced and evaluated alongside two commercially available antibodies. The optimized antibody combination demonstrated a detection limit of 1%, exhibiting superior sensitivity and accuracy to an existing commercial rapid kit. Furthermore, the developed kit showed shorter analysis time and higher cost efficiency, significantly improving its applicability in field settings. These advancements highlight the potential of this rapid immunoassay kit not only for domestic and international market penetration, but also as a critical tool for advancing GMO detection technologies.
1. 서 론
2. 재료 및 방법
2.1. 샘플 준비
2.2. 금 나노입자 합성
2.3. Dynamic Light Scattering
2.4. Zeta Potential 측정
2.5. 항체-골드 중합체 제조
2.6. 항체-골드 중합체를 이용한 면역크로마토그래피기반 lateral flow assay (LFA)
2.7. CP4 Strip test
2.8. CP4 EPSPS rapid kit 분석시간 설정
2.9. CP4 EPSPS rapid kit 민감도 시험
3. 결과 및 고찰
3.1. 금 나노입자 합성
3.2. Dynamic Light Scattering 측정
3.3. Zeta-potential 측정
3.4. CP4 strip test (pair test 결과)
3.5. CP4 EPSPS strip test 특이도 확인
3.6. CP4 strip test 최적화 조건
3.7. CP4 EPSPS rapid kit & 상용화 키트 특이도 검증
3.8. CP4 EPSPS rapid kit 분석시간 설정
3.9. CP4 EPSPS rapid kit 민감도 시험
4. 결 론
적 요
CRediT authorship contribution statement
Declaration of Competing Interest
사 사
REFERENCES
