With intensifying anthropogenic stress, incidences of extreme bioturbation have increased throughout coastal vegetation ecosystems worldwide. However, ecosystem consequences of extreme bioturbation within coastal vegetations remain poorly understood. Burrowing shrimps (infraorders Gebiidea and Axiidea) are pervasive bioturbators in seagrass ecosystems, spanning temperate to tropical regions. To date, empirical investigations about their effects on seagrass ecosystem properties are few and sparse. We conducted a series of field surveys and experiments in Geoje, South Korea to assess 1) whether burrowing shrimp Upogebia major could serve as an important bioturbating agents in intertidal seagrass Zostera japonica, 2) the relationship between bioturbation intensity and seagrass vegetation characteristics, and 3) effects of extreme shrimp bioturbation on the persistence of transplanted seagrass. Z. japonica served as an important habitat for U. major, with an average of 102 burrows m-2 found inside the vegetation compared to 266 burrows m-2 in adjacent mudflats. Increasing burrow density was associated with declining seagrass % cover, belowground biomass, and shoot density, particularly during the fall season when shrimp bioturbation activity peaked. No vegetation occurred in areas with >280 burrows m-2, suggesting a potential density threshold for seagrassshrimp co-existence. When Z. japonica was transplanted into low (~16 m-2), high (~277 m-2), and extreme high (~634 m-2) burrow-density areas, the latter two groups showed significantly faster vegetation losses. Overall, these findings suggest that: 1) U. major might serve as an important bioturbating agent in intertidal seagrass habitats across the southern coast of Korea, and 2) this species might pose a threat to seagrass resilience at high population densities.
The long-tailed goral (Naemorhedus caudatus) is classified as an endangered species in South Korea due to population declines driven by human development and habitat fragmentation. This study aimed to investigate genetic characteristics and microsatellite genotypes in long-tailed goral populations using fecal samples collected from Uljin and Samcheok regions, which are geographically important habitats for the distribution and dispersal of this species in South Korea. The mean expected heterozygosity (HE) and observed heterozygosity (HO) across 10 microsatellite loci were 0.600 and 0.461 for the Uljin population and 0.644 and 0.571 for the Samcheok population, respectively. These values indicate a moderate level of genetic diversity compared to other closely related species. Genetic differentiation between the two populations had a low level, while a moderate gene flow was observed. The genetic evidence obtained in this study suggests that these two populations share a similar genetic pool, resulting in a low level of genetic differentiation between them. Therefore, they should be considered as one continuous population. Results of this study provide fundamental data and valuable genetic insights for long-term management of the long-tailed goral population in Uljin and Samcheok regions.
Springtails (class Collembola) play a crucial role in soil ecosystems. They are commonly used as standard species in soil toxicity assessments. According to the ISO 11267 guidelines established by the International Organization for Standardization (ISO), Allonychiurus kimi uses adult survival and juvenile production as toxicity assessment endpoint. Conventional toxicity assessment methods require manually counting adults and larvae under a microscope after experiments, which is time-consuming and laborintensive. To overcome these limitations, this study developed a model using YOLOv8 to detect and count both adults and juveniles of A. kimi. An AI model was trained using a training dataset and evaluated using a validation dataset. Both training and validation datasets used for AI model were created by picturing plate images that included adults and larvae. Statistical comparison of validation dataset showed no significant difference between manual and automatic counts. Additionally, the model achieved high accuracies (Precision=1.0, Recall=0.95 for adults; Precision=0.95, Recall=0.83 for juveniles). This indicates that the model can successfully detect objects. Additionally, the system can automatically measure body areas of individuals, enabling more detailed assessments related to growth and development. Therefore, this study establishes that AI-based counting methods in toxicity assessments with offer high levels of accuracy and efficiency can effectively replace traditional manual counting methods. This method significantly enhances the efficiency of large-scale toxicity evaluations while reducing researcher workload.
The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene derived from Agrobacterium sp. strain CP4 is known to confer herbicide resistance. It has been extensively utilized in the development of living modified organism (LMO) over the past 25 years. With increasing importation of LMO agricultural products, there is a growing need for rapid and reliable detection methods for detecting the CP4 EPSPS protein. In this study, a rapid immunoassay kit based on the lateral flow assay (LFA) principle was developed to detect herbicide-resistant crops. Using recombinant CP4 EPSPS protein developed by the National Institute of Ecology, two in-house antibodies were produced and evaluated alongside two commercially available antibodies. The optimized antibody combination demonstrated a detection limit of 1%, exhibiting superior sensitivity and accuracy to an existing commercial rapid kit. Furthermore, the developed kit showed shorter analysis time and higher cost efficiency, significantly improving its applicability in field settings. These advancements highlight the potential of this rapid immunoassay kit not only for domestic and international market penetration, but also as a critical tool for advancing GMO detection technologies.
This study aimed to investigate the vascular flora of Baekunsan Mountain (255.5 m), located on Yeongjongdo Island in Jung-gu, Incheon Metropolitan City, through 14 field surveys conducted between March 2021 and April 2024, along with a re-examination of voucher specimens collected in a previous study. As a result, a total of 478 taxa, comprising 430 species, nine subspecies, and 39 varieties across 289 genera and 96 families, were identified in the area. Of these, 33 taxa were newly confirmed through specimen re-identification. Three endemic taxa native to the Korean Peninsula were documented. Additionally, three taxa listed on the IUCN red list were found; one Near Threatened (NT) and two Least Concern (LC). The survey also recorded 37 floristic target plants, including 10 taxa of grade III, six taxa of grade II, and 21 taxa of grade I. A total of 49 alien plants were identified, with a naturalization rate of 10.3% and an urbanization index of 12.5%, both showing an increase compared to past surveys. This rise is likely due to the continued development and anthropogenic disturbances in the surrounding areas. Six taxa of ecosystem-disturbing plants were found including four newly observed species, such as Ambrosia artemisiifolia, Ambrosia trifida, Aster pilosus, and Lactuca scariola, in addition to previously recorded species, such as Humulus japonicus and Rumex acetosella. Given that the Yeongjongdo Island is a geographically isolated island with limited habitat space and is subject to ongoing development plans, the area is considered highly vulnerable to environmental change. Therefore, the findings of this study are expected to serve as foundational data for monitoring biodiversity trends and formulating strategies for conservation and management of native plant species in Baekunsan Mountain and its surroundings.
Spirodela polyrhiza (L.) has been known as greater duckweed or great duckmeat. It is native inhabited in Korea. It is considered as a rich source of primary metabolites including protein, carbohydrates, and fats. Thus, it is considered as an alternative food source for the future. In addition, it has a strong phytoremediation capacity to remove various environmental pollutants, especially inorganic elements and pesticides. With a variety of duckweed’s application, there is an urgent need to develop a cultivation method for a sustainable supply of S. polyrhiza. In this study, an indoor vertical farm has been introduced to optimize duckweed cultivation. Indoor cultivated S. polyrhiza showed about 2-fold higher fresh weight than outdoor cultivated duckweed. Contents of inorganic elements were also significantly reduced in indoor cultivated S. polyrhiza. Especially, lead (Pb), cadmium (Cd), and arsenic (As) were approximately 10-fold decreased in indoor cultivated duckweed. On the other hand, contents of proteins and fats were significantly increased in indoor cultivated S. polyrhiza, while carbohydrates were found more in outdoor cultivated S. polyrhiza. Increasing N content in a homemade nutrition solution also enhanced fresh and dried weights of S. polyrhiza by about 1.8-fold in comparison with other commercial nutrition solutions. Proliferation rate (%) was doubled every 24 hours in this indoor vertical farm, indicating the accomplishment of a sustainable supply for S. polyrhiza. Further studies need to be undertaken to cultivate other duckweeds such as Wolffia arrhiza and Lemna minor using the same indoor farming system.