논문 상세보기
pp.1-5
본 연구는 고능력 한우 공란우의 이용효율을 극대화하기 위하여 반복 과배란처리가 수정란회수율에 미치는 영향을 알아보고자 수행하였다 공란우의 선발은 DNA marker에 의한 고급육을 선발하였다. 선발된 공란우는 Folltropin-V와 PG를 병용 처리하여 과배란을 유도하였고, FSH 처리 6회째 PG를 처리하여 발정을 유도하였다. 발정유도된 공란우의 수정은 12시간 간격으로 3회, 1회당 2 straw식 인공수정을 실시하였다. 수정 후 7~8일째 비외과
The purpose of this study was carried out to determine the possibility of continued embryo collection by repeatable superovulation from a Hanwoo elite cow, who was already estimated to an elite cow by DNA analysis. The donor cow was superovulated with Folltropin-V and PG combination method for 5 times, 2 months interval during 10 months. The estrus cows were inseminated with frozen semen 3 times 12 hrs interval. Embryo collection from superovulated cows was flushed non-surgical method at 7 to 8 days after artificial insemination. The superovulated cow was collected from 1 to 5 times as total (14, 4, 10, 6, 9 embryos; total 30 embryos) and transferable embryos (7, 4, 10, 3, 6; MeanS.E : 6.02.7 embryos). Seventeen recipients were transferred 24 embryos and then detected to pregnant 10 recipients after embryo transfer (58.8%; 10/17 recipients) The results indicated that the possibility of repeatable superovulation from elite donor cow for embryo transfer could be confirmed in this study, although the replicated number was not enough.
