논문 상세보기
NCSU 23 배양액에 첨가된 Insulin-Like Growth Factor-1(IGF-1)이 돼지 수정란의 체외발육에 미치는 영향 KCI 등재
Effect of Insulin-Like Growth Factor-1(IGF-1) Supplemented to NCSU 23 Medium on Preimplantation Development of Porcine Embryos
pp.219-225
본 연구는 돼지 체외수정란의 배양시스템의 최적화를 위하여 수행되었다. 기본배양액으로는 NC-SU-23을 사용하였으며, 실험 1에서는 IGF-I이 각각 0, 1, 10, 100 ng/의 농도로 첨가된 배양액에서 배양하여 분할율, 배반포 발달율과총 세포수를 확인하였다. 농도 경사에 따른 분할율은 차이가 없었으나(65.5%~69.5%), 배반포 발달율에 있어서는 10 ng/의 농도로 첨가하였을 경우가 가장 높았다(P<0.05). 실험 2는 배 아 유전자 활성
This experiments were conducted to examine the effect of Insulin-Like Growth Factor-1 (IGF-1) on preimplantation development of porcine IVF embryos. Cumulus-oocyte complexes (COCs) were matured and fertilized in vitro, and their development was monitored for 168h pi(post-insemination). In Experiment 1, embryos were cultured in NCSU23 supplemented with various concentration of IGF-1(0, 1, 10, 100 ng/). At 168h, blastocysts' cell number of inner cell mass and trophectoderm were counted. More(P<0.05) blastocysts were cultured in the medium supplemented with 10ng/m1 of IGF-1 and total cell number was the highest when embryos were cultured(P<0.05) in the same IGF-1 concentration. In Experiment 2, one-cell embryos were cultured in medium supplemented as follows: I) no supplementation; 2) without IGF-1 for 48 h pi(post-insemination) and with IGF-1 for subsequent 120 h: 3) IGF-t for 168 h; 4) with IGF-1 for 48 h and without IGF-1 for 120 h. Compared with other treatment groups, a significant increase in the proportion 2-cell embryos(P<0.05) and blastocyst(P<0.05) were obtained when cultured in medium supplemented with IGF-1 throughout in vitro culture for 168 h. As results, blastocysts cell number was the highest when oocytes were cultured in medium supplemented with 10 ng/ IGF-I throughout culture Period. However no significant difference was found on developmental competence when IGF-I was added at 4-cell stage embryos. In conclusion, 10 ng/ml of ICF-1 supplemented in NCSU-23 medium enhances porcine IVP embryo development to the blastocyst stage, but does not significantly increase cell number of blastocysts.
